Transcriptome Responses to Combinations of Stresses in Arabidopsis
Transcriptome Responses to Combinations of Stresses in Arabidopsis
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DOI：
10.1104/pp.112.210773
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发表时间：
2013-04-01
期刊：
影响因子：
7.4
通讯作者：
Mundy, John
中科院分区：
文献类型：
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作者：
Rasmussen, Simon;Barah, Pankaj;Mundy, John
生物和非生物胁迫限制了农业产量，植物往往同时受到多种胁迫。高温和干旱或低温和强光等逆境的组合对作物的表现和产量有着深远的影响。因此，划定的调控网络和代谢途径，应对单一和多重并发应力是育种和工程作物胁迫耐受性所需的。许多研究已经描述了响应于单一胁迫的转录组变化。然而，植物暴露于胁迫因子的组合可能需要激动或拮抗反应或可能与相应的单一胁迫反应无关的反应。为了分析这样的反应，我们最初比较了转录组的变化，在10个拟南芥（拟南芥）生态型使用冷，热，高光，盐，鞭毛蛋白处理作为单一的胁迫因素，以及它们的双重组合。这表明，响应于双重胁迫的约61%的转录组变化不能从对单一胁迫处理的响应中预测。它还表明，植物之间的潜在拮抗反应的优先级只有5%至10%的响应转录本。这表明，植物已经进化到能够科普各种压力，因此，可以培育出能够承受这些压力的植物。此外，使用一个子集的数据从哥伦比亚和兰茨贝格直立生态型，我们划定了共表达网络模块响应单一和组合的压力。
Biotic and abiotic stresses limit agricultural yields, and plants are often simultaneously exposed to multiple stresses. Combinations of stresses such as heat and drought or cold and high light intensity have profound effects on crop performance and yields. Thus, delineation of the regulatory networks and metabolic pathways responding to single and multiple concurrent stresses is required for breeding and engineering crop stress tolerance. Many studies have described transcriptome changes in response to single stresses. However, exposure of plants to a combination of stress factors may require agonistic or antagonistic responses or responses potentially unrelated to responses to the corresponding single stresses. To analyze such responses, we initially compared transcriptome changes in 10 Arabidopsis (Arabidopsis thaliana) ecotypes using cold, heat, high-light, salt, and flagellin treatments as single stress factors as well as their double combinations. This revealed that some 61% of the transcriptome changes in response to double stresses were not predictable from the responses to single stress treatments. It also showed that plants prioritized between potentially antagonistic responses for only 5% to 10% of the responding transcripts. This indicates that plants have evolved to cope with combinations of stresses and, therefore, may be bred to endure them. In addition, using a subset of this data from the Columbia and Landsberg erecta ecotypes, we have delineated coexpression network modules responding to single and combined stresses.