PROGESTERONE INHIBITION OF ESTROGEN-RECEPTOR REPLENISHMENT IN OVINE ENDOMETRIUM
PROGESTERONE INHIBITION OF ESTROGEN-RECEPTOR REPLENISHMENT IN OVINE ENDOMETRIUM
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DOI：
10.1095/biolreprod17.3.412
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发表时间：
1977-01-01
影响因子：
3.6
通讯作者：
STORMSHAK, F
中科院分区：
文献类型：
--
作者：
KOLIGIAN, KB;STORMSHAK, F
本文研究了雌二醇和黄体酮对去卵巢母羊子宫内膜细胞质和细胞核雌激素受体总浓度的影响。母羊（每组4只）类固醇治疗7 d：对照组（无），雌二醇-17 - β。（E2，植入物54 mg）、孕酮（P4, 15 mg，每日2次）、雌二醇和孕酮（E2 + P4）。用交换法和与[3H]雌二醇孵育1 h后测定雌激素受体的浓度。通过交换法测定[3H]雌二醇与总细胞质和核受体结合的浓度在e2处理的母羊子宫内膜中（10.40 .+-）更高（P < 0.05）。7.27和0.23 .+-。0.06 fmol femtomoles / .mu。P4-和E2 + P4处理动物子宫内膜的DNA含量(1.62 .+-。0.26和0.13。0.02 fmol / .mu。g DNA)。平均81 +-。在所有处理组母羊子宫内膜孵育期间，1%与可用细胞质受体结合的[3H]雌二醇被转运到细胞核。e2处理动物的子宫内膜（1.85 .+-）中特异性结合于细胞质并在孵育期间转移至细胞核的[3H]雌二醇浓度趋于更高（P = 0.06）。0.43 fmol / .mu。对照组、p4处理组和E2 + p4处理组母羊子宫内膜的DNA含量(总平均值1.04 +-。0.19 fmol / .mu。g DNA)。同样，体外易位处理后的母羊子宫内膜中结合在细胞质中的标记雌二醇浓度更高(0.49 .+-。0.10 fmol / .mu。对照组、P4-组和E2 + P4处理组母羊子宫内膜的DNA含量(总平均值为0.26 .+-。0.06 fmol / .mu。g DNA)。这些数据表明黄体酮通过降低细胞质雌激素受体的浓度而拮抗子宫内雌激素的结合。黄体酮的抑制作用似乎不包括抑制雌激素受体复合体向细胞核的易位。
A study was conducted to examine the influence of estradiol and progesterone on total cytoplasmic and nuclear estrogen receptor concentrations in endometrium of ovariectomized ewes. Steroid treatment of ewes (4 per group) for 7 days was as follows: Control (none), estradiol-17.beta. (E2, 54 mg implant), progesterone (P4, 15 mg twice daily) and estradiol and progesterone (E2 + P4). Concentrations of estrogen receptor were determined by exchange assay and after incubation of tissue with [3H]estradiol for 1 h. As determined by exchange assay [3H]estradiol bound to total cytoplasmic and nuclear receptors was greater (P < 0.05) in endometrium of E2-treated ewes (10.40 .+-. 7.27 and 0.23 .+-. 0.06 fmol[femtomoles]/.mu.g DNA, respectively) than in endometrium of control, P4- and E2 + P4-treated animals (1.62 .+-. 0.26 and 0.13 .+-. 0.02 fmol/.mu.g DNA, respectively). An average of 81 .+-. 1% of the [3H]estradiol bound to available cytoplasmic receptors was translocated to the nucleus during incubation of endometrium of ewes from all treatment groups. The concentration of [3H]estradiol specifically-bound in the cytoplasm and translocated to the nucleus during incubation tended to be greater (P = 0.06) in endometrium of the E2-treated animals (1.85 .+-. 0.43 fmol/.mu.g DNA) than in endometrium of ewes in the control, P4-treated and E2 + P4-treated groups (overall mean, 1.04 .+-. 0.19 fmol/.mu.g DNA). Similarly, the concentration of labeled estradiol bound in the cytoplasm after in vitro translocation was greater in endometrium of E2-treated ewes (0.49 .+-. 0.10 fmol/.mu.g DNA) than in endometrium of ewes in the control, P4- and E2 + P4-treated groups (overall mean, 0.26 .+-. 0.06 fmol/.mu.g DNA). These data suggest that progesterone antagonizes estrogen binding in the uterus by decreasing the concentration of cytoplasmic estrogen receptor. The suppressive action of progesterone does not appear to involve inhibition of estrogen-receptor complex translocation to the nucleus.