Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis
Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis
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DOI：
10.1093/carcin/bgh321
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发表时间：
2005-02-01
期刊：
影响因子：
4.7
通讯作者：
Trosko, JE
中科院分区：
文献类型：
--
作者：
Tai, MH;Chang, CC;Trosko, JE
Oct3/4基因是POU家族的一种转录因子，在胚胎干细胞和肿瘤细胞中特异表达，但在分化组织的细胞中不表达。有了分离成人干细胞的能力，就有可能测试Oct3/4基因在成人干细胞中的表达，并测试干细胞致癌的理论。利用抗体和聚合酶链式反应，我们检测了人乳腺、肝脏、胰腺、肾脏、间充质细胞和胃干细胞，癌细胞系HeLa和MCF-7以及人、狗和大鼠肿瘤中Oct4的表达。结果表明，成人干细胞、永生化的非肿瘤细胞、肿瘤细胞和细胞系表达Oct4，而分化细胞不表达Oct4。Oct4在人皮肤表皮基底层的少数细胞中表达。数据表明，成体干细胞维持Oct4的表达，这与干细胞致癌的假说一致。
The Oct3/4 gene, a POU family transcription factor, has been noted as being specifically expressed in embryonic stem cells and in tumor cells but not in cells of differentiated tissues. With the ability to isolate adult human stem cells it became possible to test for the expression of Oct3/4 gene in adult stem cells and to test the stem cell theory of carcinogenesis. Using antibodies and PCR primers we tested human breast, liver, pancreas, kidney, mesenchyme and gastric stem cells, the cancer cell lines HeLa and MCF-7 and human, dog and rat tumors for Oct4 expression. The results indicate that adult human stem cells, immortalized non-tumorigenic cells and tumor cells and cell lines, but not differentiated cells, express Oct4. Oct4 is expressed in a few cells found in the basal layer of human skin epidermis. The data demonstrate that adult stem cells maintain expression of Oct4, consistent with the stem cell hypothesis of carcinogenesis.