Fabrication, Characterization, and Cytotoxicity of Thermoplastic Polyurethane/Poly(lactic acid) Material Using Human Adipose Derived Mesenchymal Stromal Stem Cells (hASCs)
Fabrication, Characterization, and Cytotoxicity of Thermoplastic Polyurethane/Poly(lactic acid) Material Using Human Adipose Derived Mesenchymal Stromal Stem Cells (hASCs)
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DOI：
10.3390/polym10101073
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发表时间：
2018-10-01
期刊：
影响因子：
5
通讯作者：
Marycz, Krzysztof
中科院分区：
文献类型：
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作者：
Lis-Bartos, Anna;Smieszek, Agnieszka;Marycz, Krzysztof
热塑性聚氨酯（TPU）和聚乳酸是生物医学应用所需的生物相容性和可降解的合成聚合物。采用溶剂浇铸法制备了TPU+PLA物理共混组织工程基质。制备以下类型的聚合物共混物：（TPU+PLA）7：3、（TPU+PLA）6：4、（TPU+PLA）4：6和（TPU+PLA）3：7。采用各种方法来表征这些聚合物的性质：表面性质，如形态（扫描电子显微镜），润湿性（测角法），和粗糙度（轮廓分析）。对所得聚合物共混物进行了亲水性和疏水性分析、热重分析（TGA）和差示扫描量热法（DSC）。拉伸试验表明，共混物表现出广泛的机械性能。使用源自脂肪组织的人多能基质细胞（hASC）测试聚合物的细胞毒性。体外实验表明，（TPU+PLA）3：7基质是最具细胞相容性的生物材料。在（TPU+PLA）3：7上培养的细胞具有适当的形态、生长模式，并且通过增加的增殖和代谢活性来区分。此外，似乎（TPU+PLA）3：7生物材料显示出抗凋亡特性。在这些基质上培养的hASC具有降低的Bcl-α表达和增加的Bcl-2表达。该研究证明了基于（TPU+PLA）共混物产生生物相容性支架形式的可行性，所述共混物具有应用于组织工程的潜力。
Thermoplastic polyurethane (TPU) and poly(lactic acid) are types of biocompatible and degradable synthetic polymers required for biomedical applications. Physically blended (TPU+PLA) tissue engineering matrices were produced via solvent casting technique. The following types of polymer blend were prepared: (TPU+PLA) 7:3, (TPU+PLA) 6:4, (TPU+PLA) 4:6, and (TPU+PLA) 3:7. Various methods were employed to characterize the properties of these polymers: surface properties such as morphology (scanning electron microscopy), wettability (goniometry), and roughness (profilometric analysis). Analyses of hydrophilic and hydrophobic properties, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) of the obtained polymer blends were conducted. Tensile tests demonstrated that the blends exhibited a wide range of mechanical properties. Cytotoxicity of polymers was tested using human multipotent stromal cells derived from adipose tissue (hASC). In vitro assays revealed that (TPU+PLA) 3:7 matrices were the most cytocompatible biomaterials. Cells cultured on (TPU+PLA) 3:7 had proper morphology, growth pattern, and were distinguished by increased proliferative and metabolic activity. Additionally, it appeared that (TPU+PLA) 3:7 biomaterials showed antiapoptotic properties. hASC cultured on these matrices had reduced expression of Bax-alpha and increased expression of Bcl-2. This study demonstrated the feasibility of producing a biocompatible scaffold form based on (TPU+PLA) blends that have potential to be applied in tissue engineering.