NON-VIRAL OLIGONUCLEOTIDES AT THE 5' TERMINUS OF CYTOPLASMIC INFLUENZA VIRAL MESSENGER-RNA DEDUCED FROM CLONED COMPLETE GENOMIC SEQUENCES
NON-VIRAL OLIGONUCLEOTIDES AT THE 5' TERMINUS OF CYTOPLASMIC INFLUENZA VIRAL MESSENGER-RNA DEDUCED FROM CLONED COMPLETE GENOMIC SEQUENCES
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DOI：
10.1016/0092-8674(80)90486-9
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
LAI, CJ
中科院分区：
文献类型：
--
作者：
DHAR, R;CHANOCK, RM;LAI, CJ
获得了含有细胞质病毒mRNA和基因组病毒RNA序列的流感病毒DNA克隆。对5个独立分离的病毒DNA片段的末端核苷酸序列分析表明，另外的寡核苷酸与病毒mRNA转录物的5”末端共价连接。这些额外核苷酸的序列在相同基因和不同基因的DNA克隆中也有所不同。这些插入物的长度也各不相同，从6-14个核苷酸不等。这些序列的异质性表明它们最初来源于细胞RNA分子。这些发现提供了细胞RNA序列用于启动流感病毒mRNA转录感染[犬肾]细胞的证据。此外，v[病毒信使]RNA两端的序列在克隆的pFV 88中完全表达，表明克隆的DNA包含完整的血凝素病毒基因编码。
Influenza viral DNA clones containing sequences derived from cytoplasmic viral mRNA and genomic viral RNA were obtained. Sequence analysis of terminal nucleotides of 5 independently isolated viral DNA segments showed that additional oligonucleotides were covalently linked to the 5'' terminus of viral mRNA transcripts. The sequences of these additional nucleotides varied among DNA clones of the same gene and of different genes as well. These inserts also varied in length, ranging from 6-14 nucleotides. The heterogeneity of these sequences suggests that they were derived originally from cellular RNA molecules. These findings provide evidence that cellular RNA sequences are used to prime influenza viral mRNA transcription in infected [canine kidney] cells. In addition, the sequences at both termini of v[viral messenger]RNA were fully represented in clone pFV 88, indicating that the cloned DNA contained the complete viral gene coding for the hemagglutinin.