Functionalized nanoporous silicas for the immobilization of penicillin acylase
Functionalized nanoporous silicas for the immobilization of penicillin acylase
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DOI：
10.1016/j.apsusc.2004.06.080
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发表时间：
2004-10-15
影响因子：
6.7
通讯作者：
Zhao, XS
中科院分区：
文献类型：
--
作者：
Chong, ASM;Zhao, XS
自20世纪90年代以来，具有均匀孔径和有序结构的纳米多孔二氧化硅材料引起了研究人员越来越多的兴趣。以非离子型三嵌段共聚物P123为模板剂，在酸性条件下，采用共缩合法将有机硅烷与大孔纳米多孔材料SBA-15进行功能化。通过使用5种有机硅烷，即3-氨丙基三乙氧基硅烷（APTES）、3-巯丙基三甲氧基硅烷（MPTMS）、苯基三甲氧基硅烷（PTMS）、乙烯基三乙氧基硅烷（VTES）和4-（三乙氧基甲硅烷基）丁腈（TSBN），证明了该功能化，其改变了二氧化硅材料的表面性质，使该材料成为用于固定生物分子的有希望的载体。功能化的SBA-15材料表现出长程有序的尺寸范围从66到90埃的二维六边形孔阵列，如小角X射线散射（SAXS），透射电子显微镜（TEM），和物理吸附技术所证明的。通过元素分析和固态核磁共振（NMR）技术，实现了在0.5-2.6 mmol/g范围内的各种有机硅烷密度。与纯硅SBA-15相比，功能化材料显示出更好的固定化青霉素酰化酶（PA）的性能。这种改善被认为是由于增强的表面疏水性和官能团与酶的静电相互作用。(C)2004 Elsevier B. V.保留所有权利。
Nanoporous silica materials with uniform pore size and ordered structure have drawn growing interest of researchers since 1990s. A large-pore nanoporous material, SBA-15, was functionalized with organosilanes by co-condensation method in the presence of nonionic triblock copolymer P123 as a template under acidic conditions. The functionalization was demonstrated by using five organosilanes, namely 3-aminopropyltriethoxysilane (APTES), 3-mercaptopropyltrimethoxysilane (MPTMS), phenyltrimethoxysilane (PTMS), vinyltriethoxysilane (VTES), and 4-(triethoxysilyl)butyronitrile (TSBN), which modified the surface properties of the silica materials, enabling the materials to be a promising support for immobilization of biological molecules. The functionalized SBA-15 materials exhibited long-range ordering of two-dimensional hexagonal pore arrays of size ranging from 66 to 90 Angstrom as demonstrated by small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM), and physical adsorption techniques. A variety of organosilane density in the range of 0.5-2.6 mmol/g was achieved as revealed by elemental analysis and solid-state nuclear magnetic resonance (NMR) techniques. The functionalized materials displayed improved properties for immobilization of penicillin acylase (PA) in comparison with pure-silica SBA-15. Such improvement is believed to be due to the enhanced surface hydrophobicity and electrostatic interactions of the functional groups with the enzyme. (C) 2004 Elsevier B.V. All rights reserved.