Shope papillomavirus transcription in benign and malignant rabbit tumors.
Shope papillomavirus transcription in benign and malignant rabbit tumors.
复制标题
肖普乳头瘤病毒在良性和恶性兔肿瘤中的转录。
DOI：
10.1016/0042-6822(85)90058-3
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Faras,AJ
中科院分区：
文献类型：
--
作者：
Phelps,WC;Leary,SL;Faras,AJ
用北方印迹技术分析Shope乳头瘤病毒诱发的家兔和家兔良性和恶性肿瘤中病毒特异性RNA的存在。野生棉尾兔产生病毒的良性肿瘤显示含有两种主要的RNA种类，长度约为5000和3000个碱基，起源于早期区域，包括L1和L2晚期开放阅读框架的大部分。非生产性肿瘤，包括野生兔癌，良性疣和原发性和转移性癌的家兔一致地显示含有两种主要的病毒特异性RNA种类，2400和1400碱基的长度。这些转录本完全位于病毒基因组的早期区域。此外，转移性肿瘤的Southern印迹分析表明，病毒DNA仅保留在染色体外，显然未重排，支持先前上皮恶性肿瘤的发现。
Shope papillomavirus induced benign and malignant tumors from both wild and domestic rabbits were analyzed by the Northern blotting technique for the presence of viral-specific RNAs. Virus-producing benign tumors of wild cottontail rabbits are shown to contain two major RNA species approximately 5000 and 3000 bases in length that originate in the early region and include the majority of the L1 and L2 late open reading frames. Non-productive tumors including wild-rabbit carcinomas, and benign warts and primary and metastatic carcinomas of domestic rabbits uniformly are shown to contain two major viral-specific RNA species, 2400 and 1400 bases in length. These transcripts map entirely within the early region of the viral genome. In addition, Southern blot analysis of metastatic tumors indicates that the viral DNA remains exclusively extrachromosomal and apparently unrearranged supporting previous findings with epithelial malignancies.