Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition
Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition
复制标题
DOI：
--
复制
发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Zou Hua-wen;Wang Xiaohan;Hu Conglin;C. Jinsong;Zhang Xiuhai;Luo Chang;Yu Rong;Wu Zhongyi
中科院分区：
文献类型：
--
作者：
Zou Hua-wen;Wang Xiaohan;Hu Conglin;C. Jinsong;Zhang Xiuhai;Luo Chang;Yu Rong;Wu Zhongyi
干旱是影响世界农作物产量的重要限制因素之一。AtCBF4 （C-repeat binding factor 4）是CBF/DREB1（脱水响应元件结合蛋白1）转录因子的同源物，在抗旱过程中发挥重要作用。本文报道了在拟南芥RD29A启动子调控下表达AtCBF4的转基因玉米（自交系Mo17）的功能特性。测定了相对含水量（RWC）、脯氨酸（脯氨酸）和丙二醛（MDA）含量等与抗旱性有关的形态生理性状。结果表明，AtCBF4在玉米中的表达显著提高了玉米的抗旱性。脱水胁迫下，AtCBF4转基因植株L3、L4和L8表现出较野生型较低的细胞膜损伤（减少21-24%）、较高的相对含水量（增加7-12%）、较高的总可溶性糖（增加7-29%）、较高的脯氨酸含量（增加23-37%）、较好的生长发育、较大的生物量和较高的籽粒产量（增加20-25%）。在PEG条件下，转基因品系的萌发指数和耐旱性指数均显著提高。这些结果强烈表明AtCBF4的表达可以提高玉米的抗旱性。
Drought is one of the significant limiting factors affecting crops yields in the world. AtCBF4 (C-repeat binding factor 4) is a homolog of the CBF/DREB1 (dehydration-responsive element-binding protein 1) transcription factors that plays important roles during drought tolerance. Here, we report the functional characterization of transgenic maize (inbred line Mo17) expressing AtCBF4 under the control of RD29A (responsive to dehydration 29A) promoter from Arabidopsis. Some morphological and physiological traits related to drought tolerance such as relative water content (RWC), proline and MDA (malondialdehyde) content, etc. were measured. The results show that expression of AtCBF4 in maize significantly enhanced drought tolerance. Under dehydration stress, AtCBF4 transgenic plants L3, L4 and L8 showed lower cell membrane damage (decreased 21-24%), higher relative water content (increased 7-12%), higher total soluble sugars (increased 7-29%), higher proline content (increased 23-37%), better growth and development, greater biomass and higher grain yield (increased 20-25%), compared to wild type. Furthermore, the transgenic lines kept significantly higher germination index and drought tolerance index under PEG condition. These results strongly indicate that expression of AtCBF4 could increase drought tolerances in maize.