Hormesis in high-throughput screening of antibacterial compounds in E coli
Hormesis in high-throughput screening of antibacterial compounds in E coli
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DOI：
10.1177/0960327109358917
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Nascarella, Marc A.
中科院分区：
文献类型：
--
作者：
Calabrese, Edward J.;Hoffmann, George R.;Nascarella, Marc A.
本文在高通量研究中使用11种浓度（具有两倍浓度间隔）评估了1888种抗菌剂在大肠杆菌中低于毒理学阈值的响应。该数据集具有重要的优势，如对照品的低变异性（2%-3% SD）、所有威尔斯孔的重复测量和内置复制。在低于毒性阈值的浓度下，细菌生长显著大于对照组，与激素浓度反应一致。这些研究结果，沿着发表的文献分析和补充评价的浓度-响应模型预测的低浓度的影响，酵母，表明缺乏支持的广泛和历史上接受的阈值模型的浓度低于毒性阈值的反应。
This article assesses the response below a toxicological threshold for 1888 antibacterial agents in Escherichia coli, using 11 concentrations with twofold concentration spacing in a high-throughput study. The data set had important strengths such as low variability in the control (2%-3% SD), a repeat measure of all wells, and a built-in replication. Bacterial growth at concentrations below the toxic threshold is significantly greater than that in the controls, consistent with a hormetic concentration response. These findings, along with analyses of published literature and complementary evaluations of concentration-response model predictions of low-concentration effects in yeast, indicate a lack of support for the broadly and historically accepted threshold model for responses to concentrations below the toxic threshold.