Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion
Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion
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DOI：
10.1016/j.canlet.2016.05.028
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发表时间：
2016-08-28
期刊：
影响因子：
9.7
通讯作者：
Komiyama, Kazuo
中科院分区：
文献类型：
--
作者：
Mikami, Yoshikazu;Fukushima, Atsushi;Komiyama, Kazuo
分泌性白细胞蛋白酶抑制剂（SLPI）是一种丝氨酸蛋白酶抑制剂，可减少炎症期间的组织破坏。最近的一份报告揭示了SLPI在口腔癌细胞中的高水平表达。此外，SLPI过表达上调肺癌细胞的转移。另一方面，基质金属蛋白酶（MMP）是参与细胞外基质降解的蛋白酶。SLPI和MMPs作为肿瘤侵袭过程的加速剂参与其中;然而，它们的确切作用尚未完全了解。了解肿瘤侵袭的机制需要考虑微环境因素影响的模型。在一个这样的体外模型中，不同的癌细胞已经显示出以高度不同的模式侵入肌瘤组织。我们使用这种肌瘤模型，因为它提供了一个更自然的基质样环境，研究SLPI在肿瘤侵袭中的作用。我们的研究结果表明，该模型提供了一个相关的矩阵肿瘤侵袭的研究，SLPI是重要的口腔癌Ca 9 -22细胞的侵袭与MMPs。此外，使用生物信息学分析，我们已经确定了候选人作为参与SLPI介导的肿瘤侵袭的关键分子。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that diminishes tissue destruction during inflammation. A recent report revealed high levels of SLPI expression in the oral carcinoma cell. In addition, overexpression of SLPI up-regulates metastasis in lung carcinoma cells. On the other hand, matrix metalloproteinases (MMPs) are proteinases that participate in extracellular matrix degradation. SLPI and MMPs are involved as accelerators of the tumor invasion process; however, their exact roles are not fully understood. Understanding the mechanism of tumor invasion requires models that take the effect of microenvironmental factors into account. In one such in vitro model, different carcinoma cells have been shown to invade myoma tissue in highly distinct patterns. We have used this myoma model, as it provides a more natural stroma-like environment, to investigate the role of SLPI in tumor invasion. Our results indicate that the model provides a relevant matrix for tumor invasion studies, and that SLPI is important for the invasion of oral carcinoma Ca9-22 cells in conjunction with MMPs. Furthermore, using bioinformatics analysis, we have identified candidates as key molecules involved in SLPImediated tumor invasion. (C) 2016 Elsevier Ireland Ltd. All rights reserved.