Structural organization of the human MS4A gene cluster on Chromosome 11q12
Structural organization of the human MS4A gene cluster on Chromosome 11q12
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DOI：
10.1007/s002510100339
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发表时间：
2001-07-01
期刊：
影响因子：
3.2
通讯作者：
Tedder, TF
中科院分区：
文献类型：
--
作者：
Liang, YH;Buckley, TR;Tedder, TF
CD20、高亲和力的IgE受体β链(Fc Epsilon RI Beta)和HTm4是由造血细胞表达的结构相关的细胞表面蛋白。最近发现了16个新的人类和小鼠基因，编码这个新生蛋白家族的新成员，我们称之为跨膜4A基因家族，至少有12个亚组(MS4A1-MS4A12)。在目前的研究中，我们鉴定了另外三个人类MS4A基因：MS4A4E、MS4A6E和MS4A10。除MS4A6E含有两个跨膜结构域外，所有家族成员都有至少四个潜在的跨膜结构域，以及由不同外显子编码的N-末端和C-末端细胞质结构域。此外，目前发现的12个MS4A基因具有共同的结构特征和相似的内含子/外显子剪接边界，并沿着类似于染色体11q12的600kb区域聚集。与其他MS4A基因相比，MS4A4E、MS4A6E和MS4A10转录本很少见，在造血细胞和大多数非淋巴组织中都没有检测到。在MS4A6E基因中发现了序列多态性，在MS4A4A、MS4A5、MS4A6A和MS4A7基因中观察到了常见的剪接变体。因此，MS4A家族目前包括24个不同的人类和老鼠基因。与CD20和Fc epsilon RI beta一样，其他10个人类MS4A家族成员很可能是参与不同细胞系信号转导的寡聚细胞表面复合体的组成部分。
CD20, the high-affinity IgE receptor beta chain (Fc epsilon RI beta), and HTm4 are structurally related cell surface proteins expressed by hematopoietic cells. Recently, 16 novel human and mouse genes were identified that encode new members of this nascent protein family that we have named the membrane-spanning 4A gene family, with at least 12 subgroups (MS4A1-MS4A12). In the current study, we identified three additional human MS4A genes: MS4A4E, MS4A6E, and MS4A10. All family members have at least four potential transmembrane domains and N- and C-terminal cytoplasmic domains encoded by distinct exons, except MS4A6E which contains two transmembrane domains. Otherwise, the 12 currently identified MS4A genes share common structural features and similar intron/exon splice boundaries, and are clustered along an similar to 600-kb region of Chromosome 11q12. In contrast to other MS4A genes, MS4A4E, MS4A6E, and MS4A10 transcripts were rare and not detected among hematopoietic cells and most nonlymphoid tissues. Sequence polymorphisms were identified in the MS4A6E gene and common splice variants were observed for the MS4A4A, MS4A5, MS4A6A, and MS4A7 genes. Thus, the MS4A family currently includes 24 distinct human and mouse genes. Like CD20 and Fc epsilon RI beta, the 10 other human MS4A family members are likely to be components of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.