Serotonergic cells of the rat raphe nuclei express mRNA of tyrosine kinase B (trkB), the high-affinity receptor for brain derived neurotrophic factor (BDNF)
Serotonergic cells of the rat raphe nuclei express mRNA of tyrosine kinase B (trkB), the high-affinity receptor for brain derived neurotrophic factor (BDNF)
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DOI：
10.1016/s0169-328x(01)00183-8
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发表时间：
2001-09-10
期刊：
影响因子：
--
通讯作者：
Zetterström, TSC
中科院分区：
文献类型：
--
作者：
Madhav, TR;Pei, Q;Zetterström, TSC
本文研究了酪氨酸激酶B（trk B）mRNA的分布。大鼠脑内中缝核的神经元能胞体及其向背侧海马的上行投射中脑源性神经营养因子（BDNF）的高亲和力受体。以往的研究表明BDNF对多巴胺能神经元具有营养作用。在本研究中，我们提供的证据表明，多巴胺能神经元表达的BDNF，trkB的功能受体的mRNA。脑室内（i. c. v.）注射5-HT特异性神经毒素5，7-二羟色胺，其损害中缝核中的多巴胺能细胞体以及它们向背侧海马的上行投射。导致中缝背核的多巴胺能细胞体中trkB mRNA的显著丢失。相反。在背侧海马中trkB mRNA的丰度没有变化。这些发现提供了直接的证据，trkB mRNA的表达的多巴胺能神经元，并建议不同的BDNF对多巴胺能神经元的作用机制，在其细胞体和终端的投影网站的水平。(C)2001 Elsevier Science B. V.保留所有权利。
Here we have studied the distribution of mRNA for tyrosine kinase B (trkB). the high-affinity receptor for brain-derived neurotrophic factor (BDNF) amongst serotonergic cell bodies of the raphe nuclei and their ascending projections into the dorsal hippocampus in the rat brain. Previous studies have shown that BDNF has got trophic action on serotonergic neurons. In the present study, we provide evidence that serotonergic neurons express mRNA for the functional receptor of BDNF, trkB. Intracerebro-ventricular (i.c.v.) injection of the 5-HT-specific neurotoxin, 5,7-dihydroxytryptamine, which lesions serotonergic cell bodies in the raphe nuclei as well as their ascending projections into the dorsal hippocampus. caused a dramatic loss of trkB mRNA from serotonergic cell bodies of the dorsal raphe nucleus. In contrast. there was no change in the abundance of trkB mRNA within the dorsal hippocampus. These findings provide direct evidence for the expression of trkB mRNA by serotonergic neurons and suggest distinct mechanisms of action of BDNF upon serotonergic neurons at the levels of their cell bodies and terminal projection sites. (C) 2001 Elsevier Science B.V. All rights reserved.