CellAging: a tool to study segregation and partitioning in division in cell lineages of Escherichia coli
CellAging: a tool to study segregation and partitioning in division in cell lineages of Escherichia coli
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DOI：
10.1093/bioinformatics/btt194
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发表时间：
2013-07-01
期刊：
影响因子：
5.8
通讯作者：
Ribeiro, Andre S.
中科院分区：
文献类型：
--
作者：
Haekkinen, Antti;Muthukrishnan, Anantha-Barathi;Ribeiro, Andre S.
动机：大肠杆菌的细胞分裂在形态上是对称的。然而，由于不需要的蛋白质聚集体被分离到细胞两极，并且在分裂后在较老的两极积累，因此姐妹细胞的活力产生不对称。新的单分子检测技术可以观察衰老相关的过程在体内，在多个世代，告知的潜在mechanism.Results：CellAging是一种工具，自动提取信息的极性分离和分区在E。大肠杆菌，并对细胞活力。从延时，平行明场和荧光显微镜图像，它执行细胞分割，明场和荧光图像的对齐，谱系建设和极年龄确定，并计算与衰老相关的功能。我们通过分析几代细胞图像中荧光蛋白聚集体的空间分布来验证其用途。
Motivation: Cell division in Escherichia coli is morphologically symmetric. However, as unwanted protein aggregates are segregated to the cell poles and, after divisions, accumulate at older poles, generate asymmetries in sister cells' vitality. Novel single-molecule detection techniques allow observing aging-related processes in vivo, over multiple generations, informing on the underlying mechanisms.Results: CellAging is a tool to automatically extract information on polar segregation and partitioning in division of aggregates in E. coli, and on cellular vitality. From time-lapse, parallel brightfield and fluorescence microscopy images, it performs cell segmentation, alignment of brightfield and fluorescence images, lineage construction and pole age determination, and it computes aging-related features. We exemplify its use by analyzing spatial distributions of fluorescent protein aggregates from images of cells across generations.