Metal-Organic Framework-Based Insecticide and dsRNA Codelivery System for Insecticide Resistance Management.
Metal-Organic Framework-Based Insecticide and dsRNA Codelivery System for Insecticide Resistance Management.
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DOI：
10.1021/acsami.3c09074
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发表时间：
2023-10
影响因子：
9.5
通讯作者：
Chang Yu;Jiaqing Li;Zhaoyang Zhang;Mao Zong;Chuwei Qin;Ziyao Mo;Dan Sun;Disi Yang;Qinghong Zeng;Jiayin Wang;Kangsheng Ma;Jianhong Li;Hu Wan;Shun He
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文献类型：
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作者：
Chang Yu;Jiaqing Li;Zhaoyang Zhang;Mao Zong;Chuwei Qin;Ziyao Mo;Dan Sun;Disi Yang;Qinghong Zeng;Jiayin Wang;Kangsheng Ma;Jianhong Li;Hu Wan;Shun He
利用特异性双链RNA （dsRNA）靶向沉默抗性相关基因是克服害虫杀虫剂抗性的一种有吸引力的策略。然而，通过以植物运输的dsRNA为食来沉默害虫的靶基因仍然是一个挑战。本研究通过将吡虫啉和dsNlCYP6ER1包封在沸石咪唑酸框架-8 （ZIF-8）纳米颗粒内，设计了一种杀虫剂与dsRNA共递送体系，以提高褐飞虱（statl）对吡虫啉的敏感性。得到的吡虫啉/dsNlCYP6ER1@ZIF-8具有良好的单分散性，平均粒径为195 nm，表面带正电荷。生存曲线结果显示，吡虫啉和imidacloprid@ZIF-8处理组的成活率分别为82%和62%，而吡虫啉/dsNlCYP6ER1@ZIF-8处理组的成活率仅为8%。盆栽实验表明，吡虫啉/dsNlCYP6ER1@ZIF-8处理组的成活率明显低于吡虫啉处理组，从54%下降到24%。NlCYP6ER1的表达鉴定和ZIF-8的荧光跟踪表明，ZIF-8可以通过水稻向昆虫共传递dsRNA和杀虫剂。安全性评价结果表明，dsNlCYP6ER1@ZIF-8纳米颗粒对家蚕具有良好的生物相容性和生物安全性。该dsRNA和杀虫剂共递送系统可在未来扩展到其他具有潜在抗性问题的杀虫剂，极大地促进害虫抗性管理的发展。
Targeted silencing of resistance-associated genes by specific double-stranded RNA (dsRNA) is an attractive strategy for overcoming insecticide resistance in insect pests. However, silencing target genes of insect pests by feeding on dsRNA transported via plants remains challenging. Herein, a codelivery system of insecticide and dsRNA is designed by encapsulating imidacloprid and dsNlCYP6ER1 within zeolitic imidazolate framework-8 (ZIF-8) nanoparticles to improve the susceptibility of Nilaparvata lugens (Stål) to imidacloprid. With an average particle size of 195 nm and a positive surface charge, the derived imidacloprid/dsNlCYP6ER1@ZIF-8 demonstrates good monodispersity. Survival curve results showed that the survival rates of N. lugens treated with imidacloprid and imidacloprid@ZIF-8 were 82 and 62%, respectively, whereas, in the imidacloprid/dsNlCYP6ER1@ZIF-8 treatment group, the survival rate of N. lugens is only 8%. Pot experiments demonstrate that the survival rate in the imidacloprid/dsNlCYP6ER1@ZIF-8 treatment group was much lower than that in the imidacloprid treatment group, decreasing from 54 to 24%. The identification of NlCYP6ER1 expression and the fluorescence tracking of ZIF-8 demonstrate that ZIF-8 can codeliver dsRNA and insecticide to insects via rice. Safety evaluation results showed that the dsNlCYP6ER1@ZIF-8 nanoparticle had desirable biocompatibility and biosafety to silkworm. This dsRNA and insecticide codelivery system may be extended to additional insecticides with potential resistance problems in the future, greatly enhancing the development of pest resistance management.