Genetic and phenotypic variations of isolates of shrimp Taura syndrome virus found in Penaeus monodon and Metapenaeus ensis in Taiwan
Genetic and phenotypic variations of isolates of shrimp Taura syndrome virus found in Penaeus monodon and Metapenaeus ensis in Taiwan
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DOI：
10.1099/vir.0.80132-0
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发表时间：
2004-10-01
影响因子：
3.8
通讯作者：
Kou, GH
中科院分区：
文献类型：
--
作者：
Chang, YS;Peng, SE;Kou, GH
在刀新对虾（Tw2KMeTSV 分离株）、斑节对虾（Tw2KPmTSV 分离株）和凡纳滨对虾（Tw02LvTSV 分离株）中发现了不同的桃拉综合征病毒（TSV）分离株。这三个分离株的核苷酸序列分析揭示了 TSV 结构蛋白（衣壳蛋白前体）基因 orf2 的差异。 TSV ORF2 氨基酸序列比较和系统发育分析表明，这 3 个台湾分离株与夏威夷分离株 HI94TSV 之间存在较密切的亲缘关系。在自然和实验感染 Tw2KPmTSV 分离株的斑节对虾样本中，病毒被包含在内，并且虾没有表现出感染的临床症状。然而，当斑节对虾受到 Tw2KMeTSV 分离株的攻击时，病毒会自由复制。 Tw2KMeTSV 分离株的 ORF2 氨基酸序列在四个位置上与 Tw2KPmTSV 分离株不同，这些差异可能解释了它们的表型差异，至少在它们在特定宿主中复制的能力方面。
Distinct Taura syndrome virus (TSV) isolates were found in Metapenaeus ensis (isolate Tw2KMeTSV), Penaeus monodon (isolate Tw2KPmTSV) and Litopenaeus vannamei (isolate Tw02LvTSV). Nucleotide sequence analysis of these three isolates revealed differences in the TSV structural protein (capsid protein precursor) gene orf2. TSV ORF2 amino acid sequence comparison and phylogenetic analysis suggested a comparatively close relationship between these three Taiwanese isolates and the Hawaiian isolate HI94TSV. In P. monodon specimens that were naturally and experimentally infected with the Tw2KPmTSV isolate, the virus was contained and shrimps showed no clinical signs of infection. However, when P. monodon was challenged with the Tw2KMeTSV isolate, the virus replicated freely. The ORF2 amino acid sequence of the Tw2KMeTSV isolate differed from that of isolate Tw2KPmTSV in four positions and these differences may account for their phenotypic differences, at least in terms of their ability to replicate in specific hosts.