Molecular diffusion in tissue-engineered cartilage constructs: Effects of scaffold material, time, and culture conditions
Molecular diffusion in tissue-engineered cartilage constructs: Effects of scaffold material, time, and culture conditions
复制标题
DOI：
10.1002/jbm.b.30053
复制
发表时间：
2004-08-15
影响因子：
3.4
通讯作者：
Guilak, F
中科院分区：
文献类型：
--
作者：
Leddy, HA;Awad, HA;Guilak, F
扩散可能是组织工程软骨中大分子运输的主要机制，通过扩散提供足够的营养供应可能是细胞增殖和细胞外基质产生所必需的。本研究的目的是测量组织工程软骨构建物的扩散率，作为支架材料、培养条件和培养时间的函数。四种不同大小的荧光葡聚糖的扩散系数通过荧光恢复光漂白后的组织工程软骨构建体中，用人脂肪来源的干细胞或在海藻酸盐、琼脂糖、明胶或纤维蛋白支架上的脱细胞构建体，在软骨形成或对照条件下培养1或28天。人工软骨的扩散率比天然软骨大得多。从第1天到第28天，脱细胞构建体的扩散率增加了62%，而在软骨形成和对照培养中，细胞构建体的扩散率分别下降了42%和27%。细胞结构中弥散性的降低可能是由于新的基质合成和纤维蛋白和明胶支架中的细胞对基质的收缩。在脱细胞结构中扩散率的增加可能是由于支架的降解和膨胀。(C) 2004。公司。
Diffusion is likely to be the primary mechanism for macromolecular transport in tissue-engineered cartilage, and providing an adequate nutrient supply via diffusion may be necessary for cell proliferation and extracellular matrix production. The goal of this study was to measure the diffusivity of tissue-engineered cartilage constructs as a function of scaffold material, culture conditions, and time in culture. Diffusion coefficients of four different-sized fluorescent dextrans were measured by fluorescence recovery after photobleaching in tissue-engineered cartilage constructs seeded with human adipose-derived stem cells or acellular constructs on scaffolds of alginate, agarose, gelatin, or fibrin that were cultured for 1 or 28 days in either chondrogenic or control conditions. Diffusivities in the constructs were much greater than those of native cartilage. The diffusivity of acellullar constructs increased 62% from Day 1 to Day 28, whereas diffusivity of cellular constructs decreased 42% and 27% in chondrogenic and control cultures, respectively. The decrease in diffusivity in cellular constructs is likely due to new matrix synthesis, which may be enhanced with chondrogenic media, and matrix contraction by the cells in the fibrin and gelatin scaffolds. The increase in diffusivity in the acellullar constructs is probably due to scaffold degradation and swelling. (C) 2004 Wiley Periodicals. Inc.