Protein-Protein Interactions Leading to Recruitment of the Host DNA Sliding Clamp by the Hyperthermophilic Sulfolobus islandicus Rod-Shaped Virus 2
Protein-Protein Interactions Leading to Recruitment of the Host DNA Sliding Clamp by the Hyperthermophilic Sulfolobus islandicus Rod-Shaped Virus 2
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DOI：
10.1128/jvi.00636-14
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发表时间：
2014-06-01
影响因子：
5.4
通讯作者：
Krupovic, Mart
中科院分区：
文献类型：
--
作者：
Gardner, Andrew F.;Bell, Stephen D.;Krupovic, Mart
感染超嗜热古菌的病毒通常不编码 DNA 聚合酶，这引发了有关其基因组复制的问题。在这里，我们使用酵母双杂交方法评估了岛硫化叶菌杆状病毒 2 (SIRV2) 蛋白与宿主编码的增殖细胞核抗原 (PCNA)（古细菌中关键的 DNA 复制蛋白）之间的相互作用。发现五种 SIRV2 蛋白与 PCNA 相互作用，为病毒 DNA 复制的宿主复制体的招募提供了见解。
Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for viral DNA replication.