High-Throughput RAD-SNP Genotyping for Characterization of Sugar Beet Genotypes
High-Throughput RAD-SNP Genotyping for Characterization of Sugar Beet Genotypes
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DOI：
10.1007/s11105-013-0685-x
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发表时间：
2014-06-01
影响因子：
2.1
通讯作者：
Concheri, Giuseppe
中科院分区：
文献类型：
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作者：
Stevanato, Piergiorgio;Broccanello, Chiara;Concheri, Giuseppe
高通量单核苷酸多态性（SNP）基因分型提供了一种快速的方法来开发资源丰富的标记集，用于描绘遗传结构和理解分类歧视的基础。在本文中，我们提出了一个小组的192个SNP有效的基因分型甜菜使用高通量标记阵列技术，QuantStudio 12K Flex系统，再加上Taqman OpenArray技术。选择的SNPs进行了评估的遗传多样性之间的一组150个人代表15个基因型（10个人），从5个细胞质雄性不育（CMS），5个授粉，和5个商业品种。我们证明了所提出的192个SNP的面板有效地区分了所研究的基因型。与传粉者和商业品种相比，CMS之间观察到更高程度的多态性。PCoA和结构分析表明，CMS，传粉者，品种聚成三个不同的亚群。我们的研究结果表明，实用程序的192个SNPs加上TaqMan OpenArray技术作为一个广泛的一组标记的高通量SNP基因分型甜菜。
High-throughput single-nucleotide polymorphism (SNP) genotyping provides a rapid way of developing resourceful sets of markers for delineating genetic structure and for understanding the basis of the taxonomic discrimination. In this paper, we present a panel of 192 SNPs for effective genotyping in sugar beet using a high-throughput marker array technology, QuantStudio 12K Flex system, coupled with Taqman OpenArray technology. The selected SNPs were evaluated for genetic diversity among a set of 150 individuals representing 15 genotypes (10 individuals each) from five cytoplasmic male steriles (CMSs), five pollinators, and five commercial varieties. We demonstrated that the proposed panel of 192 SNPs effectively differentiated the studied genotypes. A higher degree of polymorphism was observed among the CMSs as compared to pollinators and commercial varieties. PCoA and STRUCTURE analysis revealed that CMSs, pollinators, and varieties clustered into three distinct subpopulations. Our results demonstrate the utility of the identified panel of 192 SNPs coupled with TaqMan OpenArray technology as a wide set of markers for high-throughput SNP genotyping in sugar beet.