Identification of miR414 and expression analysis of conserved miRNAs from Stevia rebaudiana.
Identification of miR414 and expression analysis of conserved miRNAs from Stevia rebaudiana.
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DOI：
10.1016/s1672-0229(11)60024-7
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发表时间：
2011-12
影响因子：
9.5
通讯作者：
Yadav, Sudesh Kumar
中科院分区：
文献类型：
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作者：
Guleria, Praveen;Yadav, Sudesh Kumar
microRNA（miRNAs）通常含有19-24个核苷酸，是真核生物重要的基因调控因子。各种计算方法的应用已经通过从可用的序列数据源预测miRNA来简化任务。在这项研究中，我们确定了一个保守的miR 414从甜菊EST序列数据的计算分析。此外，我们还通过茎环RT-PCR分析鉴定了miR 169、miR 319、miR 414、miR 164、miR 167和miR 398等6种保守的miRNAs。因此，miR 414通常使用两种方法鉴定。这些miRNAs的表达分析证实了它们在甜菊生长发育中的作用。此外，发现检测到的miRNAs靶向参与植物生长、发育、代谢和信号转导的基因。这是首次报道这些保守的miRNAs及其在甜菊中的表达的研究。
MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators. Applications of various computational approaches have simplified the task by predicting miRNAs from available sequence data sources. In this study, we identified a conserved miR414 from a computational analysis of EST sequence data available from Stevia rebaudiana. In addition, we also identified six conserved miRNAs namely miR169, miR319, miR414, miR164, miR167 and miR398 using stem-loop RT-PCR analysis. Hence, miR414 was commonly identified using both methods. The expression analysis of these miRNAs documented their roles in growth and development of Stevia. Furthermore, the detected miRNAs were found to target genes involved in plant growth, development, metabolism and signal transduction. This is the first study reporting these conserved miRNAs and their expression in Stevia.