Synchronised determination of chlorogenic acid and five flavonoids in mulberry leaves using HPLC with photodiode array detection
Synchronised determination of chlorogenic acid and five flavonoids in mulberry leaves using HPLC with photodiode array detection
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DOI：
10.3920/qas2017.1202
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发表时间：
2018-02
影响因子：
4
通讯作者：
K. Zhai;D. Hong;Shu-xiang Shi;L.-L. Liu-L.;Cao Wengen;Gui-zhen Gao;Ling-ling Shan
中科院分区：
文献类型：
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作者：
K. Zhai;D. Hong;Shu-xiang Shi;L.-L. Liu-L.;Cao Wengen;Gui-zhen Gao;Ling-ling Shan
建立了高效液相色谱-二极管阵列检测器同时测定桑葚中绿原酸和5种黄酮类化合物（芦丁、异槲皮苷、槲皮苷、槲皮素和木犀草素）的分析方法。在梯度洗脱条件下在Shim Pack VP-ODS C18上进行色谱分离（250 × 4.6 mm，粒径5 μm）柱，温度30 °C，移动的相为0.5%（v/v）磷酸水溶液和乙腈，流速0.9 ml/min（对于绿原酸、槲皮苷和木犀草素）、350 nm（对于芦丁和异槲皮苷）和365 nm（对于槲皮素）。采用最佳提取工艺对桑葚中的绿原酸和5种黄酮类化合物进行了提取，平均回收率为97.78%~ 103.24%。在线性、回收率、精密度和稳定性方面对所开发的方法进行了验证。关系……
An analytical method based on high-performance liquid chromatography separation with diode array detection was developed and validated for the synchronised determination of chlorogenic acid and five flavonoids (rutin, isoquercitrin, quercitrin, quercetin, and luteolin) in mulberry leaves (Morus spp.). Chromatographic separation was carried out under gradient elution conditions on a Shim Pack VP-ODS C18 (250 × 4.6 mm, particle size 5 μm) column at a temperature of 30 °C using a mobile phase consisting of 0.5% (v/v) phosphoric acid in water and acetonitrile at a flow rate of 0.9 ml/min. The column eluent process was best at 330 nm (for chlorogenic acid, quercitrin, and luteolin), 350 nm (for rutin and isoquercitrin), and 365 nm (for quercetin). Application of optimum extraction conditions led to extraction of chlorogenic acid and five flavonoids from mulberry leaves with mean recoveries of 97.78-103.24%. The developed method was validated in terms of linearity, recovery, precision, and stability. The relati...