Mode of action of hypoglycemic agents. V. Studies with phenethylbiguanide in isolated perfused rat liver.
Mode of action of hypoglycemic agents. V. Studies with phenethylbiguanide in isolated perfused rat liver.
复制标题
降血糖药的作用方式。
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
H. Lardy
中科院分区：
文献类型：
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作者：
D. E. Cook;J. B. Blair;H. Lardy
在离体灌注大鼠肝脏中，苯乙基双胍抑制乳酸和二羟基丙酮产生葡萄糖的速率，但对木糖醇产生葡萄糖没有抑制作用。在所研究的所有底物中，苯乙基双胍降低了果糖6-磷酸和α-酮戊二酸的稳态浓度，降低了ATP:ADP比率，降低了柠檬酸循环活性，并导致线粒体和细胞质氧化还原状态进一步降低。所提出和讨论的结果被解释为表明苯乙基双胍通过间接机制影响肝脏糖异生。
Abstract In isolated perfused rat liver, phenethylbiguanide inhibits the rate of glucose production from both lactate and dihydroxyacetone, but not from xylitol. With all substrates studied, phenethylbiguanide decreases the steady state concentrations of fructose 6-phosphate and α-ketoglutarate, decreases the ATP:ADP ratio, decreases citric acid cycle activity, and causes both the mitochondrial and cytosolic oxidation-reduction states to be more reduced. The results presented and discussed are interpreted to indicate that phenethylbiguanide influences hepatic gluconeogenesis by an indirect mechanism.