Streptobacillus notomytis sp nov., isolated from a spinifex hopping mouse (Notomys alexis Thomas, 1922), and emended description of Streptobacillus Levaditi et al. 1925, Eisenberg et al. 2015 emend
Streptobacillus notomytis sp nov., isolated from a spinifex hopping mouse (Notomys alexis Thomas, 1922), and emended description of Streptobacillus Levaditi et al. 1925, Eisenberg et al. 2015 emend
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DOI：
10.1099/ijsem.0.000654
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发表时间：
2015-12-01
影响因子：
2.8
通讯作者：
Kaempfer, Peter
中科院分区：
文献类型：
--
作者：
Eisenberg, Tobias;Glaeser, Stefanie P.;Kaempfer, Peter
1979年，从一只刺尾蚤跳鼠的心脏中分离到一种多形、革兰氏阴性、杆状、吲哚、氧化酶和过氧化氢酶阴性、不产芽孢、不运动的细菌（Notomys alexis托马斯，1922），败血症，并作为念珠状链杆菌储存在西澳大利亚州南珀斯动物卫生实验室的菌株保藏中心（阿勒370-1）以及CCUG 12425。16 S rRNA基因序列分析表明，该菌株与念珠状链杆菌（Streptobacillusmoniliformis）模式株的同源性为99.4%，与香港链杆菌（Streptobacillushongkongensis）模式株的同源性为95.6%，与猫链杆菌（Streptobacillusfelis）模式株的同源性为99.0%。rpoB、groEL和recA核苷酸和氨基酸序列分析也支持菌株阿勒370-1（T）与念珠状链杆菌、香港链杆菌和猫链杆菌的明显区分。菌株阿勒370-1（T）与念珠状链杆菌DSM 12112（T）的平均核苷酸同源性为87.16%.考虑到与密切相关的物种相比，酶活性和脂肪酸的特征非常相似，生理数据证实了菌株阿勒370-1（T）属于细毛菌科。在链杆菌属中，阿勒370-1（T）菌株也能与念珠状链杆菌、香港链杆菌和猫链杆菌的模式菌株明确分离。从日本无症状黑鼠中分离的另外两个菌株（KWG 2和KWG 24）与阿勒370-1（T）高度相似。根据这些数据，我们提出了新物种Streptobacillus notomytis sp. nov.，模式菌株阿勒370-1（T）（=CCUG 12425（T）=DSM 100026（T）=CCM 8593（T）=EF 12425（T））。
A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 % sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 % sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1(T) from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1(T) and Streptobacillus moniliformis DSM 12112(T). Physiological data confirmed the allocation of strain AHL 370-1(T) to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus, isolate AHL 370-1(T) could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1(T). On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL 370-1(T) (=CCUG 12425(T)=DSM 100026(T)=CCM 8593(T)=EF 12425(T)).