Telomere length and anaemia in old age: results from the Newcastle 85-plus Study* and the Leiden 85-plus Study
Telomere length and anaemia in old age: results from the Newcastle 85-plus Study* and the Leiden 85-plus Study
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DOI：
10.1093/ageing/afr048
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发表时间：
2011-07-01
期刊：
影响因子：
6.7
通讯作者：
Gussekloo, Jacobijn
中科院分区：
文献类型：
--
作者：
Den Elzen, Wendy P. J.;Martin-Ruiz, Carmen;Gussekloo, Jacobijn
背景资料：血细胞中端粒长度的减少与多种年龄相关疾病的风险增加有关，并被广泛认为是衰老的一般生物标志物。因此，了解这种关联的程度和局限性是很重要的。我们在两个独立的队列中调查了端粒长度和贫血之间的关系，并事先期望将贫血添加到端粒减少是风险因素的条件列表中。参与者和方法：本研究被纳入纽卡斯尔85岁以上研究和莱顿85岁以上研究，这两项研究是对英国纽卡斯尔和北泰恩赛德（n = 749）和莱顿的居民进行的基于人口的研究，荷兰（n = 658）85岁及以上。从新鲜全血（纽卡斯尔）和外周血单核细胞样本（莱顿）中分离高分子量DNA。端粒长度通过定量实时聚合酶链反应测量为端粒模板相对于单个基因的丰度。根据世界卫生组织的标准定义贫血。结果：在两项研究中，观察到贫血参与者和非贫血参与者的端粒长度中位数没有差异（纽卡斯尔：2，846 bp（四分位距（IQR）2，433 - 3，630）对比2，920 bp（IQR 2，425 - 3，570），P = 0.63;莱顿：4，136 bp（IQR 3，879 - 4，428）vs 4，167 bp（IQR 3，893 - 4，501），P = 0.41）。端粒长度也没有与任何其他血液参数在男性和women.Conclusions：在其他年龄相关疾病，端粒长度与贫血或任何其他血液参数在老年人在一般人群中。
Background: reduced telomere length in blood cells has been associated with increased risk of multiple age-related diseases and is widely regarded as a general biomarker of ageing. Therefore, it is important to know both the extent and limitations of this association. We investigated the relation between telomere length and anaemia in two independent cohorts, with the prior expectation of adding anaemia to the list of conditions for which telomere reduction is a risk factor.Participants and methods: the present study is embedded in the Newcastle 85-plus Study and Leiden 85-plus Study, two population-based studies of inhabitants of Newcastle and North Tyneside, UK (n = 749) and Leiden, the Netherlands (n = 658) aged 85 and over. High-molecular-weight DNA was isolated from full fresh blood (Newcastle) and peripheral blood mononuclear cells samples (Leiden). Telomere length was measured as abundance of telomeric template versus a single gene by quantitative real-time polymerase chain reaction. Anaemia was defined according to World Health Organization criteria.Results: in both studies, no differences in median telomere length were observed between participants with anaemia and participants without anaemia (Newcastle: 2,846 bp (interquartile range (IQR) 2,433-3,630) versus 2,920 bp (IQR 2,425-3,570), P = 0.63; Leiden: 4,136 bp (IQR 3,879-4,428) versus 4,167 bp (IQR 3,893-4,501), P = 0.41). Telomere length also did not correlate with any other haematological parameter in both men and women.Conclusions: in contrast to other age-related diseases, telomere length is not associated with anaemia or any other haematological parameter in older individuals in the general population.