Size and mechanical stability of norovirus capsids depend on pH: a nanoindentation study
Size and mechanical stability of norovirus capsids depend on pH: a nanoindentation study
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DOI：
10.1099/vir.0.021212-0
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发表时间：
2010-10-01
影响因子：
3.8
通讯作者：
Donath, E.
中科院分区：
文献类型：
--
作者：
Cuellar, J. L.;Meinhoevel, F.;Donath, E.
使用原子力显微镜对诺如病毒样颗粒进行成像。通过纳米压痕在 2 至 10 的 pH 值范围内探测病毒样颗粒 (VLP) 的机械稳定性。该范围包括诺如病毒生命周期中自然环境的 pH 值。VLP 对压痕的抵抗力在酸性和中性 pH 值下保持不变。杨氏模量约为30 MPa。在碱性 pH 值下，衣壳的顺应性随着直径的增加而增加。衣壳的这种特定的 pH 依赖性机械反应可能与感染期间控制 RNA 的摄取和释放的机制有关。压力连续压痕
Norovirus-like particles were imaged using atomic force microscopy. The mechanical stability of the virus-like particles (VLPs) was probed by nanoindentation at pH values ranging from 2 to 10. This range includes pH values of the natural environment during the life cycle of noroviruses The resistance of VLPs to indentation was constant at acidic and neutral pH. The Young's modulus was of the order of 30 MPa. At basic pH the compliance of the capsid increased along with an increase in diameter. This specific pH-dependent mechanical response of the capsid may be related to mechanisms controlling uptake and release of the RNA during infection. Consecutive indentations with pressures