A SUBSTANTIAL PART OF GLUT-1 IN CRUDE MEMBRANES FROM MUSCLE ORIGINATES FROM PERINEURIAL SHEATHS
A SUBSTANTIAL PART OF GLUT-1 IN CRUDE MEMBRANES FROM MUSCLE ORIGINATES FROM PERINEURIAL SHEATHS
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DOI：
10.1152/ajpendo.1992.262.5.e721
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发表时间：
1992-05-01
影响因子：
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通讯作者：
VINTEN, J
中科院分区：
文献类型：
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作者：
HANDBERG, A;KAYSER, L;VINTEN, J
用免疫细胞化学方法研究了GLUT-1和GLUT-4在大鼠骨骼肌冰冻切片中的分布。 强烈的标记GLUT-1被发现在神经束膜鞘的肌内神经，而只有一个非常微弱的信号与肌膜，和标记的神经血管是不可检测的。 大部分GLUT-4反应性位于不均匀斑块中的肌细胞的外周，并且GLUT-4在血管和神经中不存在。 在股神经切片中，GLUT-1仅分布于神经束膜鞘和神经内膜血管。 GLUT-1的肌内神经束膜鞘中的总GLUT-1在肌肉中的贡献，确定从分离的混合物的匀浆的切除的神经和肌肉的粗膜的免疫印迹。 GLUT-1的回收率随着神经添加量线性增加，并且计算出来自肌内神经束膜鞘的GLUT-1占来自比目鱼肌或红腓肠肌的膜部分中GLUT-1含量的约60%。 其余40%的GLUT-1可能来源于肌膜。
The distribution of GLUT-1 and GLUT-4 in cryosections of rat skeletal muscles was investigated immunocytochemically. Intense labeling of GLUT-1 was found in the perineurial sheaths of intramuscular nerves, whereas only a very faint signal was associated with the sarcolemma, and labeling of extraneurial vessels was not detectable. The majority of the GLUT-4 reactivity was located at the periphery of muscle cells in nonuniform patches, and GLUT-4 was absent in vessles and nerves. In sections of femoral nerve GLUT-1 was confined to the perineurial sheath and endoneurial vessels. The contribution of GLUT-1 from intramuscular perineurial sheaths to total GLUT-1 in a muscle was determined from immunoblots of crude membranes isolated from mixtures of homogenates of excised nerves and muscles. The recovery of GLUT-1 increased linearly with the amount of nerve added, and it was calculated that GLUT-1 from intramuscular perineurial sheaths accounted for approximately 60% of the GLUT-1 content in a membrane fraction from soleus muscle or red gastrocnemius. The remaining 40% of GLUT-1 is likely to originate from the sarcolemma.