Semiquantitative immunohistochemical analysis for hypoxia in human tumors
Semiquantitative immunohistochemical analysis for hypoxia in human tumors
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DOI：
10.1016/s0360-3016(00)01505-4
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发表时间：
2001-02-01
影响因子：
7
通讯作者：
Varia, MA
中科院分区：
文献类型：
--
作者：
Raleigh, JA;Chou, SC;Varia, MA
目的：本研究的目的是开发一个半定量评分系统，用于测量缺氧在人类肿瘤的免疫组化marker approach.Methods和材料：18例患者诊断为鳞状细胞癌的子宫颈或头颈部静脉输注盐酸哌莫硝唑溶液，剂量为0.5 gm/m2。24小时后，将每个肿瘤的平均4个活检组织固定在福尔马林中，加工成石蜡块并切片。对组织切片进行免疫染色以确定是否存在哌莫硝唑加合物。捕获免疫染色的显微图像（x200）并通过标准图像分析进行定量。已知缺氧量范围为> 0%至5%、> 5%至15%、> 15%至30%和>30%的图像分别被赋予+1、+2、+3和+4的分数。然后，三名观察者使用该校准的评分系统以盲法分析肿瘤切片中的缺氧。通过校准的、半定量的、用于鳞状细胞癌中缺氧的免疫组化测定，获得了良好的观察者间重现性。经过校准的，半定量分析显示出作为简化通过免疫组织化学技术定量人类肿瘤缺氧的方法的前景。(C)2001 Elsevier Science Inc.
Objective: The goal of this study was to develop a semiquantitative scoring system for measuring hypoxia in human tumors by an immunohistochemical marker approach.Methods and Materials: Eighteen patients diagnosed with squamons cell carcinoma of the uterine cervix or head add neck were infused intravenously with a solution of pimonidazole hydrochloride at a dose of 0.5 gm/m(2). Twenty-four hours later, four biopsies on average from each tumor were fixed in formalin, processed into paraffin blocks, and sectioned. Tissue sections were immunostained for the presence of pimonidazole adducts, Microscopic images (x200) of immunostaining were captured and quantitated by standard image analysis. images with known amounts of hypoxia spanning ranges of > 0% to 5%, > 5% to 15%, > 15% to 30%, and >30% were assigned scores of +1, +2, +3, and +4, respectively, Three observers then used this calibrated scoring system to analyze hypoxia in tumor sections in a blinded fashion.Results: Excellent interobserver reproducibility was obtained with the calibrated, semiquantitative, immunohistochemical assay for hypoxia in squamous cell carcinomas.Conclusion: The calibrated, semiquantitative assay shows promise as an approach to simplifying the quantitation of human tumor hypoxia by immunohistochemical techniques. (C) 2001 Elsevier Science Inc.