Deoxyribonucleic acid base sequence relatedness among members of the yeast genus Kluyveromyces
Deoxyribonucleic acid base sequence relatedness among members of the yeast genus Kluyveromyces
复制标题
克鲁维酵母属酵母菌中脱氧核糖核酸碱基序列的相关性
DOI：
10.1099/00207713-37-4-371
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
H. Phaff
中科院分区：
文献类型：
--
作者：
G. B. Fuson;H. L. Presley;H. Phaff
脱氧核糖核酸（DNA）碱基组成和DNA碱基序列的相关性比较用于种划分在克鲁维酵母属。碱基组成值将该属的成员分为三组。通过使用DNA/DNA复性实验比较碱基序列进一步细分组。鉴定了两个DNA同源组。第一组包括马克思克鲁维酵母、脆壁克鲁维酵母、保加利亚克鲁维酵母、钩孢克鲁维酵母、威肯克鲁维酵母和三种无性型（开菲尔假丝酵母、假热带假丝酵母和乳脂圆酵母）;该组成员的DNA碱基序列互补性≥90%。第二组由乳酸克鲁维酵母、范努登克鲁维酵母、果蝇克鲁维酵母和菜豆孢克鲁维酵母组成;这些酵母的不同对共享64%至98%的DNA序列。这两个组仅彼此有较远的亲缘关系（DNA碱基序列互补性≤15%）。其他克鲁维酵母属物种似乎是独特的，不密切相关的两个同源组或彼此。从DNA比较中推断出的关系与其他工作者从外切β-葡聚糖酶的免疫学比较和同工酶分析中推断出的关系一致，但仅与基于交配研究的分类学安排部分一致。我们提出了以下物种的识别：克鲁维酵母aestuarii，克鲁维酵母africanus，克鲁维酵母blattae，克鲁维酵母delphensis，克鲁维酵母dobzhanskii，克鲁维酵母。lactis（syn. K. drosophilarum，K. phaseolosporus和K. vanudenii）、洛氏克鲁维酵母（Kluyveromyces lodderi）、克鲁维酵母（K.马克思主义（syn. K. bulgaricus，K. cicerisporus，K. fragilis和K. wikenii）、法菲克鲁维酵母（Kluyveromyces phaffii）、多孢克鲁维酵母（Kluyveromyces polysporus）、耐热克鲁维酵母（Kluyveromyces thermotolerans）、瓦氏克鲁维酵母（Kluyveromyces waltii）和威克汉克鲁维酵母（Kluyveromyces wickerhamii）。
Deoxyribonucleic acid (DNA) base composition and DNA base sequence relatedness comparisons were used for species delineation in the genus Kluyveromyces. Base composition values separated the members of the genus into three groups. The groups were further subdivided by comparing base sequences by using DNA/DNA renaturation experiments. Two DNA homology groups were identified. The first group included Kluyveromyces marxianus, Kluyveromyces fragilis, Kluyveromyces bulgaricus, Kluyveromyces cicerisporus, Kluyveromyces wikenii, and three anamorphs (Candida kefyr, Candida pseudotropicalis, and Torula cremoris); the members of this group exhibited ≥90% DNA base sequence complementarity. The second group consisted of Kluyveromyces lactis, Kluyveromyces vanudenii, Kluyveromyces drosophilarum, and Kluyveromyces phaseolosporus; various pairs of these yeasts shared 64 to 98% of their DNA sequences. The two groups were only distantly related to each other (≤15% DNA base sequence complementarity). The other Kluyveromyces species appear to be unique, not being closely related to either of the two homology groups or to one another. Relationships deduced from comparisons of DNAs agreed well with those deduced by other workers from immunological comparisons of exo-β-glucanases and from isoenzyme analysis but were only in partial agreement with a taxonomic arrangement made on the basis of mating studies. We propose recognition of the following species: Kluyveromyces aestuarii, Kluyveromyces africanus, Kluyveromyces blattae, Kluyveromyces delphensis, Kluyveromyces dobzhanskii, K. lactis (syn. K. drosophilarum, K. phaseolosporus, and K. vanudenii), Kluyveromyces lodderi, K. marxianus (syn. K. bulgaricus, K. cicerisporus, K. fragilis, and K. wikenii), Kluyveromyces phaffii, Kluyveromyces polysporus, Kluyveromyces thermotolerans, Kluyveromyces waltii, and Kluyveromyces wickerhamii.