Prion protein gene polymorphisms in healthy and scrapie-affected sheep in Greece
Prion protein gene polymorphisms in healthy and scrapie-affected sheep in Greece
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DOI：
10.1099/vir.0.19520-0
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发表时间：
2004-02-01
影响因子：
3.8
通讯作者：
Papadopoulos, O
中科院分区：
文献类型：
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作者：
Billinis, C;Psychas, V;Papadopoulos, O
研究人员对来自 16 个受瘙痒病影响的希腊羊群的 216 只本地杂交羊和来自健康羊群的 210 只 Chios 和 Karagouniko 纯种羊进行了朊病毒蛋白 (PrP) 基因中与瘙痒病相关的多态性分析。在本病例对照研究的 216 只羊中，96 只为临床病例，25 只为亚临床病例（安乐死时无症状，但组织病理学和/或 ELISA 检测蛋白酶抗性 PrP 呈阳性）和 95 只健康对照（所有评估均为阴性）。通过变性梯度凝胶电泳、随后进行 RFLP 和测序来确定密码子 136、154 和 171 的多态性。临床和亚临床瘙痒病与基因型 ARQ/ARQ（110 只羊中的 88 只属于该基因型）、ARQ/TRQ（13 只中的 9 只）、ARQ/AHQ（38 只中的 115 只）和 VRQ/VRQ（17 只中的 9 只）相关。临床病例的组织病理学病变更为严重。仅在健康对照羊中检测到基因型 ARQ/ARR（26 只羊）、ARQ/ARK（7 只羊）、AHQ/ARR（1 只羊）、ARH/ARH（1 只羊）和 ARR/ARH（3 只羊）。在纯种调查中，希俄斯羊中存在四种基因型（ARQ/ARQ、ARQ/TRQ、ARQ/AHQ 和 ARQ/ARR），卡拉古尼科羊中存在四种基因型（ARQ/ARQ、ARQ/AHQ、ARQ/ARR 和 ARQ/ARH）。
A total of 216 local crossbred sheep from 16 scrapie-affected Greek flocks and 210 purebred sheep of the milk breeds Chios and Karagouniko from healthy flocks were analysed for scrapie-linked polymorphisms in the prion protein (PrP) gene. Of the 216 sheep in this case-control study, 96 sheep were clinical cases, 25 subclinical cases (asymptomatic at the moment of euthanasia but positive by histopathology and/or ELISA detecting proteinase-resistant PrP) and 95 healthy controls (negative by all evaluations). Polymorphisms at codons 136, 154 and 171 were determined by denaturing gradient gel electrophoresis, followed by RFLP and sequencing. Scrapie, both clinical and subclinical, was associated with the genotypes ARQ/ARQ (88 of 110 sheep of that genotype), ARQ/TRQ (9 of 13), ARQ/AHQ (115 of 38) and VRQ/VRQ (9 of 17). Histopathological lesions were more severe in the clinical cases. Genotypes ARQ/ARR (26 sheep), ARQ/ARK (seven sheep), AHQ/ARR (one sheep), ARH/ARH (one sheep) and ARR/ARH (three sheep) were detected exclusively in healthy control sheep. In the purebred survey, four genotypes were present in the Chios sheep (ARQ/ARQ, ARQ/TRQ, ARQ/AHQ and ARQ/ARR) and four in the Karagouniko sheep (ARQ/ARQ, ARQ/AHQ, ARQ/ARR and ARQ/ARH).