EXERCISE TRAINING INCREASES GLUCOSE TRANSPORTER CONTENT IN SKELETAL-MUSCLES MORE EFFICIENTLY FROM AGED OBESE RATS THAN YOUNG LEAN RATS
EXERCISE TRAINING INCREASES GLUCOSE TRANSPORTER CONTENT IN SKELETAL-MUSCLES MORE EFFICIENTLY FROM AGED OBESE RATS THAN YOUNG LEAN RATS
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DOI：
10.2337/diabetes.41.8.920
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发表时间：
1992-08-01
期刊：
影响因子：
7.7
通讯作者：
ITAKURA, H
中科院分区：
文献类型：
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作者：
EZAKI, O;HIGUCHI, M;ITAKURA, H
大鼠骨骼肌葡萄糖摄取量随着年龄和肥胖的增加而减少，但随着慢性运动训练的增加而增加。本研究的目的是确定1个月龄的幼年瘦大鼠和12个月龄的肥胖大鼠骨骼肌中GLUT4的含量是否随着运动训练的改变而改变。在运动方面，两组大鼠均进行约1公里/天的跑台跑4wk。用免疫印迹法检测几种骨骼肌膜蛋白中GLUT4的浓度。老年大鼠腓肠肌和白色股四头肌中GLUT4的含量略有下降，但显著下降，分别为青年大鼠的73%和78%。然而，年轻和老年大鼠的比目鱼肌、足底肌和红股四头肌中GLUT4的含量没有显著差异。运动训练使老年大鼠各肌肉中GLUT4的含量增加幅度大于幼年大鼠肌肉。老年大鼠的比目鱼肌、足底肌、腓肠肌和红色股四头肌的GLUT4含量分别比安静对照组增加30%、33%、41%和27%。然而，在幼年大鼠中，运动诱导的GLUT4含量仅在足底显著增加，增幅为17%。运动老年肥胖大鼠体重下降，血浆甘油三酯和游离脂肪酸水平下降。提示老年大鼠部分骨骼肌中GLUT4含量略有下降，肥胖率升高，运动训练增加老年大鼠GLUT4含量比青年大鼠更有效，并能改善老年大鼠GLUT4含量的下降，脂代谢可能与调节肌肉中GLUT4蛋白含量有关。
Glucose uptake in rat skeletal muscles decreases with age and obesity, but increases with chronic exercise training. The purpose of our study was to determine whether the GLUT4 content in several skeletal muscles from 1-mo-old young, lean rats and 12-mo-old aged, obese rats alters with exercise training. For exercise, a treadmill run of approximately 1 km/day was made for 4 wk by both groups of rats. The concentration of GLUT4 per protein in membrane fraction from several skeletal muscles was measured by immunoblotting. The amount of GLUT4 in the gastrocnemius and white quadriceps from aged rats slightly but significantly decreased to 73% and 78% of that from young rats, respectively. However, no significant difference in GLUT4 amount in the soleus, plantaris, and red quadriceps was observed between young and aged rats. The exercise training resulted in a larger increase in the amount of GLUT4 in each muscle from aged rate than in muscles from young rats. In aged rats, GLUT4 amount increased significantly with exercise training by 30, 33, 41, and 27% in the soleus, plantaris, gastrocnemius, and red quadriceps, respectively, compared with the sedentary controls. However, in young rats, exercise-induced increase of GLUT4 amount was significant only in the plantaris, and the increase was 17%. In exercise aged, obese rats, decreases of body weight, plasma triglyceride levels, and plasma free fatty acid were also observed. These findings suggest that the amount of GLUT4 in some skeletal muscles decreases slightly in aged, obese rate, exercise training increases the amount of GLUT4 more efficiently in aged rate,than in young rats and ameliorates the GLUT4 decrease observed in aged rats, and lipid metabolism may be related to the regulation of GLUT4 protein content in muscles.