Comparison of Anopheles gambiae and Culex pipiens acetycholinesterase 1 biochemical properties
Comparison of Anopheles gambiae and Culex pipiens acetycholinesterase 1 biochemical properties
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DOI：
10.1016/j.cbpb.2008.03.008
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发表时间：
2008-07-01
影响因子：
2.2
通讯作者：
Weill, Mylene
中科院分区：
文献类型：
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作者：
Alout, Haoues;Djogbenou, Luc;Weill, Mylene
乙酰胆碱酯酶1（AChE 1）的选择不敏感发生在几种蚊用氨基甲酸酯（CX）和有机磷（OP）杀虫剂控制。在拟除虫菊酯抗药性的情况下，这些杀虫剂代表了病媒控制方案的替代方案。它们在农业中的大量使用选择了西非冈比亚按蚊的抗性种群。必须研究抗药性的演变，以防止或至少减缓抗药性蚊子在野生种群中的传播。一个.冈比亚库蚊对CX和OP杀虫剂的抗性机制与尖音库蚊相同，均与AChE 1酶的G119 S取代有关。通过比较这两个物种的耐药AChE-1，我们在这里表明，获得类似的电阻水平对10杀虫剂的两个类。此外，类似的AChE 1活性水平之间记录的两个物种的敏感或抗性蚊子。因此，属于这两个物种的酶似乎具有相同的性质。因此，我们假设，健身成本与乙酰胆碱酯酶1不敏感性在C。pipiens蚊子应该是类似于在一个。因此，可将其用于控制疟疾流行地区抗药性人群的战略。(C)2008年爱思唯尔公司All rights reserved.
Selection of insensitive acetycholinesterase 1 (AChE1) has occurred in several mosquito species controlled with carbarnate (CX) and organophosphate (OP) insecticides. In case of pyrethroid resistance, these insecticides represent an alternative for disease vector control program. Their heavy use in agriculture has selected resistant populations of Anopheles gambiae in West Africa. The evolution of resistance has to be studied to prevent, or at least slow down, the spread of resistant mosquito in wild populations. An. gambiae shares the same resistance mechanism to CX and OP insecticides as Culex pipiens, which was attributed to the G119S substitution in the AChE1 enzyme. By comparing resistant AChE-1 from both species, we show here that similar resistance levels are obtained toward 10 insecticides of both classes. Moreover, similar AChE1 activity levels are recorded between either susceptible or resistant mosquitoes of both species. Enzymes belonging to both species seem thus to share identical properties. Consequently, we hypothesize that fitness cost associated with AChE1 insensitivity in C. pipiens mosquitoes should be similar in An. gambiae and thus be used in strategies to control resistant populations where malaria is prevalent. (C) 2008 Elsevier Inc. All rights reserved.