Levels of serum and synovial fluid pyridinium crosslinks in patients with rheumatoid arthritis.
Levels of serum and synovial fluid pyridinium crosslinks in patients with rheumatoid arthritis.
复制标题
类风湿关节炎患者血清和滑液吡啶交联水平。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
H. Morii
中科院分区：
文献类型：
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作者：
Y. Furumitsu;M. Inaba;K. Yukioka;M. Yukioka;Y. Kumeda;Y. Azuma;T. Ohta;T. Ochi;Y. Nishizawà;H. Morii
目的
阐明类风湿关节炎（RA）中吡啶鎓交联的主要来源。
方法
收集75例RA患者和41例健康对照者的血清样本，并收集20例RA患者和13例骨关节炎（OA）患者的滑液（SF）样本。同时对26例RA患者进行血清和SF配对检测。通过最近开发的使用高压液相色谱法的高灵敏度测定方法测定吡啶鎓交联的水平。
结果
RA患者血清吡啶酚（PYD）和脱氧吡啶酚（DPD）水平显著高于健康对照组，且与疾病活动性和严重程度相关。RA患者的SF DPD水平显著高于OA患者，但SF PYD水平无显著差异。RA患者SF PYD、SF DPD水平与SF IL 1 β、SF IL 6水平均呈显著正相关。最后，在同一时间收集的所有配对RA样本中，SF中的PYD水平高于血清中的DPD水平，但DPD水平不高于血清中的PYD水平，每对成员之间具有显著相关性。
结论
这些观察结果表明，RA血清中PYD的增加可能主要来自受影响的关节，RA血清中DPD的增加可能更多地受到全身性骨吸收的影响。
OBJECTIVE
To elucidate the major source of pyridinium crosslinks in rheumatoid arthritis (RA).
METHODS
Serum samples were collected from 75 patients with RA and 41 healthy controls, and synovial fluid (SF) samples were collected from 20 patients with RA and 13 with osteoarthritis (OA). Paired samples of serum and SF were collected at the same time from 26 patients with RA. Levels of pyridinium crosslinks were determined by a recently developed high sensitivity assay method using high pressure liquid chromatography.
RESULTS
The levels of serum pyridinoline (PYD) and serum deoxypyridinoline (DPD) were significantly higher in patients with RA than in healthy controls, and significantly correlated with laboratory variables indicating disease activity and severity. The levels of SF DPD, but not SF PYD, were significantly higher in patients with RA than in patients with OA. The levels of SF PYD and SF DPD both showed a significantly positive correlation with those of either SF interleukin 1beta or SF interleukin 6 in patients with RA. Finally, the levels of PYD, but not DPD, were higher in SF than in serum in all paired RA samples collected at the same time, with significant correlation between the members of each pair.
CONCLUSION
These observations suggest than an increase of PYD in RA serum may originate mostly from affected joints and that an increase of DPD in RA serum may be influenced more by systemic bone resorption.