CA-2+-MG-2+ ATPASE OF MOUSE CARDIAC SARCOPLASMIC-RETICULUM IS AFFECTED BY MEMBRANE N-6 AND N-3 POLY-UNSATURATED FATTY-ACID CONTENT
CA-2+-MG-2+ ATPASE OF MOUSE CARDIAC SARCOPLASMIC-RETICULUM IS AFFECTED BY MEMBRANE N-6 AND N-3 POLY-UNSATURATED FATTY-ACID CONTENT
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DOI：
10.1093/jn/119.3.364
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发表时间：
1989-03-01
影响因子：
4.2
通讯作者：
KINSELLA, JE
中科院分区：
文献类型：
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作者：
SWANSON, JE;LOKESH, BR;KINSELLA, JE
白鼠体重18-20克，喂食含有2%红花油和10%鲱鱼、玉米或橄榄油的纯化饲料2周。与食用玉米或橄榄油相比，食用鲱鱼油可显著提高22:6（n-3）和20:5（n-3）水平，尤其是22:6(n-3)，并降低心脏肌浆网（SR）磷脂的20:4（n-6）和18:2（n-6）水平。这些脂肪酸组成的变化导致心脏SR磷脂的n-6/n-3脂肪酸比值显著降低。玉米和鲱鱼油对小鼠SR中胆碱磷脂和乙醇胺磷脂的比值分别为2.8∶0.2和1.9∶0.2。这种n-6/n-3脂肪酸比值的降低与Ca2+-Mg2+ atp酶的相对活性降低有关，也与饲喂鱼鱼油而不是橄榄油或玉米油的小鼠SR囊泡中钙运输的初始速率和最大钙吸收量降低有关。饲料脂质对心脏SR NADPH细胞色素C还原酶（EC 1.6.2.3）的比活性无显著影响。这些数据表明，对SR进行22:6（n-3）修饰可能会改变SR双层结构，从而改变SR囊泡的钙转运特性。此外，我们的研究结果表明，食用鱼油后心脏SR钙通量的减少也可能降低心肌细胞对缺血和再灌注期间可能发生的钙浓度快速变化的敏感性。
White mice, 18-20 g, were fed purified diets containing two weight percent safflower oil plus ten weight percent menhaden, corn, or olive oil for 2 wk. Menhaden oil ingestion resulted in significantly higher levels of 22:6(n-3) and 20:5(n-3), particularly 22:6(n-3), and lower levels of 20:4(n-6) and 18:2(n-6) in cardiac sarcoplasmic reticulum (SR) phospholipids than did corn or olive oil ingestion. These changes in fatty acid composition resulted in a significant decrease in the value of the n-6/n-3 fatty acid ratio of cardiac SR phospholipids. The ratio was 2.8 versus 0.2 in choline phospholipids and 1.9 versus 0.2 in ethanolamine phospholipids in SR of mice fed corn or menhaden oil, respectively. This reduction in the n-6/n-3 fatty acid ratio was associated with a lower relative activity of Ca2+-Mg2+ ATPase, and a lower initial rate of calcium transport and maximum calcium uptake in SR vesicles from mice fed menhaden oil rather than olive or corn oils. The specific activity of NADPH cytochrome C reductase (EC 1.6.2.3) of cardiac SR was not affected by dietary lipids. These data indicate that modification of SR by 22:6(n-3) may change the SR bilayer structure resulting in alteration of the calcium transport properties of SR vesicles. In addition, our results suggest that reduction of calcium flux across cardiac SR following fish oil consumption may also reduce the susceptibility of myocytes to rapid changes in calcium concentrations which may occur during ischemia and reperfusion.