Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
复制标题
在体神经活动的千赫双光子荧光显微成像
DOI：
10.1038/s41592-020-0762-7
复制
发表时间：
2020-03-02
期刊：
影响因子：
48
通讯作者：
Ji, Na
中科院分区：
文献类型：
--
作者：
Wu, Jianglai;Liang, Yajie;Ji, Na
理解大脑中的信息处理需要以高时空分辨率监测神经元活动。使用由全光学激光扫描授权的超快双光子荧光显微镜，我们以每秒3,000帧和亚微米空间分辨率成像体内神经元活动。这种成像方法使监测的超和阈下电活动下降到345 μ m以下的大脑表面在头部固定清醒mice.High-speed双光子激光扫描显微镜使用被动激光扫描仪的基础上自由空间角啁啾增强延迟实现帧速率适合电压成像在体内小鼠大脑。
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice.High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.