Genetic analysis of regulatory, promoter, and TAR regions of LTR sequences belonging to HIV type 1 non-B subtypes
Genetic analysis of regulatory, promoter, and TAR regions of LTR sequences belonging to HIV type 1 non-B subtypes
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DOI：
10.1089/aid.2005.21.949
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发表时间：
2005-11-01
影响因子：
1.5
通讯作者：
Holguín, A
中科院分区：
文献类型：
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作者：
De Arellano, ER;Soriano, V;Holguín, A
HIV-1基因表达的转录激活受序列特异性转录因子与长末端重复序列（LTR）的相互作用控制。关于不同HIV-1亚型LTR区域之间差异的信息很少。LTR序列，包括调节，增强子，启动子和TAR区域，在59个已知感染非B亚型的HIV-1感染者中进行遗传表征和比较。系统发育分析将LTR区域归于以下亚型：10A、2B、6C、1D、2 E、2F、16 G、3 J、2 H和2U。高达34%的样品是LTR/PR重组体。LTR区域揭示了不同HIV-1亚型之间的高度遗传变异性，并显示了几个亚型特异性标记，假设这些标记可以影响与细胞转录因子的相互作用，从而导致不同HIV-1进化枝之间的不同转录水平。据我们所知，这是属于J和H亚型的LTR序列的第一个特征。
Transcriptional activation of HIV-1 gene expression is controlled by the interaction of sequence-specific transcription factors with the long terminal repeat (LTR). Information about differences between LTR regions in distinct HIV-1 subtypes is scarce. LTR sequences, including regulatory, enhancer, promoter, and TAR regions, were genetically characterized and compared in 59 HIV-1-infected individuals known to be infected with non-B subtypes. Phylogenetic analyses ascribed the LTR regions to the following subtypes: 10A, 2B, 6C, 1D, 2E, 2F, 16G, 3J, 2H, and 2U. Up to 34% of the samples were LTR/PR recombinants. The LTR region revealed a high degree of genetic variability among distinct HIV-1 subtypes and showed several subtype-specific markers, which hypothetically could influence the interactions with cellular transcription factors, leading to different transcriptional levels among distinct HIV-1 clades. To our knowledge, this is the first characterization of LTR sequences belonging to subtypes J and H.