P25(RUM1) ORDERS S-PHASE AND MITOSIS BY ACTING AS AN INHIBITOR OF THE P34(CDC2) MITOTIC KINASE
P25(RUM1) ORDERS S-PHASE AND MITOSIS BY ACTING AS AN INHIBITOR OF THE P34(CDC2) MITOTIC KINASE
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DOI：
10.1016/0092-8674(95)90215-5
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发表时间：
1995-12-15
期刊：
影响因子：
64.5
通讯作者：
NURSE, P
中科院分区：
文献类型：
--
作者：
CORREABORDES, J;NURSE, P
来自裂解酵母的p25(Rum1)被证明是p34(Cdc2)/p56(Cdc13)有丝分裂酶的特异性体外抑制物。研究还表明，早期G1细胞含有p25(Rum1)，它与有丝分裂激酶结合并抑制，并将p56(Cdc13)分裂B周期蛋白维持在低水平，确保这些细胞不会过早进入有丝分裂。G2细胞中高水平的p25(Rum1)抑制了p34(Cdc2)/p56(Cdc13)激酶，从而阻止了进一步的S期，并导致了重复的DNA复制。因此，作用于p34(CDC2)的细胞周期蛋白依赖性激酶抑制因子p25(Rum1)在保证细胞周期中S期和有丝分裂的正确顺序中起着重要作用。
p25(rum1) from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34(cdc2)/p56(cdc13) mitotic kinase. It is also shown that early G1 cells contain p25(rum1), which associates with and inhibits the mitotic kinase, and maintains p56(cdc13) miotic B cyclin at a low level, ensuring that these cells do not undergo a premature lethal entry into mitosis. A high level of p25(rum1) in G2 cells inhibits the p34(cdc2)/p56(cdc13) kinase that removes the block preventing a further S phase and leads to repeated rounds of DNA replication. Thus, the cyclin-dependent kinase inhibitor p25(rum1), acting on the p34(cdc2) mitotic kinase, plays an important role in ensuring the correct sequence of S phase and mitosis during the cell cycle.