A novel pathogen-binding gC1qR homolog, FcgC1qR, in the Chinese white shrimp, Fenneropenaeus chinensis
A novel pathogen-binding gC1qR homolog, FcgC1qR, in the Chinese white shrimp, Fenneropenaeus chinensis
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中国明对虾中一种新的病原体结合 gC1qR 同源物 FcgC1qR
DOI：
10.1016/j.dci.2011.08.005
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发表时间：
2012-02-01
影响因子：
2.9
通讯作者：
Wang, Jin-Xing
中科院分区：
文献类型：
--
作者：
Li, Xin-Cang;Du, Zhi-Qiang;Wang, Jin-Xing
在脊椎动物中，补体成分C1q受体（gC1qR）的球状“头部”是一种多功能的多配体结合蛋白。然而，关于其在无脊椎动物中的功能的研究有限。本研究克隆了中国白色对虾（Fenneropenaeus chinensis）gC1qR新同源基因FcgC1qR的全长cDNA序列。半定量聚合酶链反应（PCR）检测FcgC1qR在所有检查的组织，在肠道中检测到的最高水平。Western blot检测结果表明，FcgC1qR蛋白在正常中国白色对虾的血淋巴中有表达，但在其他组织中没有表达。在表达模式研究中，定量实时PCR证实FcgC1qR的转录物在细菌（Vibrio anguillarum或Staphylococcus aureus）和白色斑点综合征病毒（white spot syndrome virus）的攻击下上调。随后，在大肠杆菌中高效表达FcgC1qR，并制备了其多克隆抗体。用Western blot检测FcgC1qR与微生物的结合，结果表明FcgC1qR能与蜡样芽孢杆菌、苏云金芽孢杆菌、S.金黄色葡萄球菌、鳗弧菌、哈维氏弧菌和白色念珠菌。FcgC1qR也被证明能够与S.金黄色葡萄球菌以浓度依赖性的方式，并且这种结合活性被多克隆抗体部分抑制。这些结果表明FcgC1qR可能参与了中国白色对虾对细菌感染的防御。(C)2011爱思唯尔有限公司保留所有权利。
In vertebrates, the globular "head" of complement component C1q receptor (gC1qR) is a versatile, multiligand binding protein. However, research on its function in invertebrates is limited. In the present study, a full-length cDNA sequence of a novel gC1qR homolog, FcgC1qR, from the Chinese white shrimp Fenneropenaeus chinensis was cloned. Semi-quantitative polymerase chain reaction (PCR) detected FcgC1qR in all examined tissues, with the highest level detected in the intestine. Western blot assay further revealed that the FcgC1qR protein was distributed in all tested tissues except the cell-free hemolymph of normal Chinese white shrimp. In the expression pattern study, quantitative real-time PCR demonstrated that the transcripts of FcgC1qR were up-regulated when challenged with bacteria (Vibrio anguillarum or Staphylococcus aureus) and white spot syndrome virus. Subsequently, FcgC1qR was over-expressed in Escherichia coli, and the polyclonal antibody was prepared with the purified recombinant protein. Microorganism binding was examined using Western blot assay, and revealed that FcgC1qR could bind to Bacillus cereus, Bacillus thuringiensis, S. aureus, V. anguillarum, Vibrio harveyi, and Candida albicans. FcgC1qR was also proven able to bind to S. aureus in a concentration-dependent manner, and this binding activity was partly inhibited by the polyclonal antibody. These results suggest that FcgC1qR may be involved in defending against bacterial infections in the Chinese white shrimp. (C) 2011 Elsevier Ltd. All rights reserved.