Development of a specific system for targeting protein to metallophilic macrophages
Development of a specific system for targeting protein to metallophilic macrophages
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DOI：
10.1073/pnas.0308490100
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发表时间：
2004-02-17
影响因子：
11.1
通讯作者：
Martinez-Pomares, L
中科院分区：
文献类型：
--
作者：
Taylor, PR;Zamze, S;Martinez-Pomares, L
The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naive mice, these CRL+ cells, metallophilic macrophages (MO) in spleen and subcapsular sinus M(P in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL+ Mphi. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mphi in an immune response in vivo.
