Atbf1 is required for the Pit1 gene early activation
Atbf1 is required for the Pit1 gene early activation
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DOI：
10.1073/pnas.0712196105
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发表时间：
2008-02-19
影响因子：
11.1
通讯作者：
Carriere, Catherine
中科院分区：
文献类型：
--
作者：
Qi, Yingchuan;Ranish, Jeffrey A.;Carriere, Catherine
增强子的功能已经被描述了大约35年，但是在哺乳动物器官发生过程中，调控输入与替代基因增强子整合的分子原理仍然不完全清楚。利用体内增强子定位和蛋白质组学方法的结合，我们已经确定了两个遥远的和不同的早期增强子，每个需要不同的转录复合物，是编码垂体谱系决定因子Pit1的基因完全激活所必需的。基因和体外分析显示，一种属于“巨型、多同源结构域和锌指家族”的转录因子Atbf1，作为两种必需增强子之一的一种新的垂体调节因子。
Enhancers have been functionally described for > 35 years, but the molecular principles underlying the integration of regulatory inputs to alternate gene enhancers used during mammalian organogenesis remain incompletely understood. Using a combination of in vivo enhancer mapping and proteomics approaches, we have established that two distant and distinct early enhancers, each requiring different transcription complexes, are required for full activation of the gene encoding the pituitary lineage determining factor, Pit1. A transcription factor belonging to the "giant, multiple-homeodomain and zinc finger family," Atbf1, serves as a novel pituitary regulator for one of the two required enhancers as shown by genetic and in vitro analysis.