Stress repressive expression of rice SRZ1 and characterization of plant SRZ gene family
Stress repressive expression of rice SRZ1 and characterization of plant SRZ gene family
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水稻SRZ1的逆境抑制表达及植物SRZ基因家族的鉴定
DOI：
10.1016/j.plantsci.2007.11.010
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发表时间：
2008-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Huang, Ji;Wang, Mei-Mei;Zhang, Hong-Sheng
从水稻中克隆了一个锌指蛋白基因，命名为SRZ 1（stress repressive zinc finger protein 1）。SRZ1编码一种具有三个C2C2型锌指基序的蛋白质，其结构与人ZNF 265锌指基序相似。SRZ1在水稻幼苗中的表达量较高，盐、冷、干旱和脱落酸胁迫显著抑制SRZ1的表达，而水杨酸和稻瘟病菌对SRZ1的表达无明显影响。SRZ1基因在烟草中的异位表达抑制了非生物胁迫相关基因的表达，包括Eschertin，NtERB10B，NtERB10C，并增加了植物对冷和盐胁迫的敏感性。检索数据库发现SRZ基因广泛分布于植物中，水稻和拟南芥中SRZ基因的表达在至少一种非生物胁迫下显著下调。这项研究表明，SRZ 1及其在植物中的近亲可能在非生物胁迫信号传导中发挥负作用，它们的下调可能对植物耐受胁迫至关重要。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
A zinc finger protein gene was cloned from rice and designated as SRZ1 (stress repressive zinc finger protein 1). SRZ1 encodes a protein with three C2C2-type zinc finger motifs that are structurally similar to human ZNF265 zinc finger motifs. SRZ1 was expressed with high level in leaves and markedly repressed by salt, cold, drought and abcisic acid stresses but not by salicylic acid and blast inoculation in rice seedlings. Ectopic expression of SRZ1 in tobacco plants repressed expression of abiotic stress-related genes including osmotin, NtERB10B, NtERB10C, and increased plant sensitivity to cold and salt stresses. Database searches showed SRZ genes were widely distributed among plant species and their expressions in rice and Arabidopsis were significantly down-regulated by at least one type of abiotic stresses. This study indicates that SRZ1 and its relatives in plants may play negative roles in abiotic stress signaling and the down-regulation of them might be crucial for plant tolerance against stresses. (c) 2007 Elsevier Ireland Ltd. All rights reserved.