Organization of the respiratory chain of Neisseria meningitidis
Organization of the respiratory chain of Neisseria meningitidis
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DOI：
10.1042/bst0340139
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发表时间：
2006-02-01
影响因子：
3.9
通讯作者：
Moir, J
中科院分区：
文献类型：
--
作者：
Deeudom, M;Rock, J;Moir, J
脑膜炎奈瑟菌利用氧和氮氧化物作为呼吸底物的能力使其能够在人类宿主的多样化环境中茁壮成长。基因组分析强调了编码cbb(3)细胞色素氧化酶、aniA亚硝酸盐还原酶基因和norB一氧化氮还原酶基因的基因。在本研究中，我们在完整细胞中使用粘噻唑作为bc(1)复合物的抑制剂，并证明了流向亚硝酸盐还原酶和细胞色素氧化酶的电子流，而不是NO还原酶，通过细胞色素bc(1)复合物。完整细胞的紫外可见分光光度法表明，氧氧化c型和b型细胞色素。亚硝酸盐氧化细胞色素仅在微生物预培养的全细胞中可见，可氧化细胞色素以b型为主。这些可能与含b-血的一氧化氮还原酶的氧化有关。亚硝酸盐以不依赖于亚硝酸盐还原酶的方式抑制氧对细胞色素的氧化，表明亚硝酸盐可能直接抑制氧化酶活性，也可能通过反硝化的中间体一氧化氮抑制氧化酶活性。
The ability of Neisseria meningitidis to utilize both oxygen and nitrogen oxides as respiratory substrates allows it to thrive in the diverse environment of the human host. Genome analysis highlighted genes encoding a cbb(3) cytochrome oxidase, the aniA nitrite reductase gene and the norB nitric oxide reductase gene. In the present study, we used myxothiazol as an inhibitor of the bc(1) complex in intact cells and demonstrated that electron flow to nitrite reductase and the cytochrome oxidase, but not NO reductase, passes via the cytochrome bc(1) complex. UV-visible spectrophotometry of intact cells demonstrated that oxygen oxidizes c-type and b-type cytochromes. oxidation of cytochromes by nitrite was only seen in microaerobically precultured whole cells, and the predominant oxidizable cytochromes were b-type. These are likely to be associated with the oxidation of a b-haem-containing nitric oxide reductase. Nitrite inhibits the oxidation of cytochromes by oxygen in a nitrite reductase-independent manner, indicating that nitrite may inhibit oxidase activity directly, as well as via the intermediate of denitrification, nitric oxide.