The CXCL12/CXCR4 autocrine loop increases the metastatic potential of non-small cell lung cancer in vitro
The CXCL12/CXCR4 autocrine loop increases the metastatic potential of non-small cell lung cancer in vitro
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DOI：
10.3892/ol.2012.960
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发表时间：
2013-01-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Hao
中科院分区：
文献类型：
--
作者：
Dai, Xiaofeng;Mao, Zhifu;Zhang, Hao
CXCL 12/CXCR 4内分泌轴已被证明在许多不同类型肿瘤的器官特异性转移中起关键作用，但CXCL 12/CXCR 4自分泌环的确切作用仍知之甚少。本研究利用基因转染技术在A549细胞中构建了CXCL 12/CXCR 4自分泌环，以评估其对非小细胞肺癌（NSCLC）转移的影响。我们的研究结果表明，CXCL 12/CXCR 4自分泌环显着促进A549细胞的运动，增殖和侵袭，提示CXCL 12/CXCR 4自分泌环在NSCLC转移中的关键作用。此外，这些发现表明，针对CXCR 4的靶向治疗应考虑待治疗NSCLC的CXCL 12表达状态，因为CXCL 12自分泌过表达的肿瘤可能更适合应用基于趋化因子的抗癌治疗。
The CXCL12/CXCR4 endocrine axis has been demonstrated to play a pivotal role in organ-specific metastasis of many different types of tumors, but the precise role of the CXCL12/CXCR4 autocrine loop remains poorly understood. In this study, we constructed a functional CXCL12/CXCR4 autocrine loop in A549 cells using a gene transfection technique to evaluate its effect on the metastasis of non-small cell lung cancer (NSCLC). Our results demonstrated that the CXCL12/CXCR4 autocrine loop significantly promoted the motility, proliferation and invasiveness of the A549 cells, suggesting a key role of the CXCL12/CXCR4 autocrine loop in NSCLC metastasis. In addition, these findings suggest that targeted therapies directed against CXCR4 should consider the CXCL12 expression status of the NSCLC to be treated, since tumors with autocrine overexpression of CXCL12 may be more suitable for the application of chemokine-based anticancer therapies.