Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm
Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm
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DOI：
10.2135/cropsci2007.08.0474
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发表时间：
2008-09-01
期刊：
影响因子：
2.3
通讯作者：
Lagudah, Evans S.
中科院分区：
文献类型：
--
作者：
Kolmer, James A.;Singh, Ravi P.;Lagudah, Evans S.
Lr 34/Yr 18成株抗性基因对持久性叶锈病（由小麦柄锈菌（Puccinia triticina Eriks）引起）有显著贡献。阻力利用简单、可靠的分子标记技术对Lr 34/Yr 18基因进行早期检测是小麦遗传学研究的一个重要进展。育种一个插入/缺失大小的变异体位于7 D号染色体上的csLV 34位点，位于与Lr 34/Yr 18双抗锈基因紧密连锁的硫酸盐转运蛋白样基因的内含子序列内，用于检查全球收集的小麦品种、地方品种和含有D基因组的二倍体和多倍体小麦亲缘种。在小麦品种中发现的两个主要等位基因大小变异体，csLV 34 a和B，在不同的小麦种植区表现出不同的变化。在Lr 34/Yr 18和csLV 34 b等位基因的存在之间观察到强关联，并且已知具有csLV 34 a等位基因的Lr 34/Yr 18的小麦品系是罕见的。除中国地方品种外，其余地方品种均以csLV 34 a型为主。只有一个大小的变异，csLV 34 a，检测到二倍体和多倍体D基因组的物种，表明csLV 34 b出现后，六倍体面包小麦合成。在来自北美和南美、CIMMYT、澳大利亚和俄罗斯的现代小麦栽培品种中，与Lr 34/Yr 18相关的csLV 34 b等位基因的谱系被追溯到20世纪初Nazareno Strampelli在意大利开发的栽培品种Mentana和Ardito。csLV 34标记在推测Lr 34/Yr 18在广泛的小麦种质中可能发生的稳健性及其在小麦育种中的实用性得到证实。
The Lr34/Yr18 adult plant resistance gene contributes significantly to durable leaf rust (caused by Puccinia triticina Eriks.) resistance. Simple and robust molecular markers that enable early detection of Lr34/Yr18 are a major advancement in wheat (Triticum aestivum L.) breeding. An insertion/deletion size variant located at the csLV34 locus on chromosome 7D within an intron sequence of a sulfate transporter-like gene tightly linked to the Lr34/Yr18 dual rust resistance gene was used to examine a global collection of wheat cultivars, landraces, and D genome-containing diploid and polyploid species of wheat relatives. Two predominant allelic size variants, csLV34a and b, found among the wheat cultivars showed disparate variation in different wheat growing zones. A strong association was observed between the presence of Lr34/Yr18 and the csLV34b allele and wheat lines known to have Lr34/Yr18 that had the csLV34a allele were rare. All landraces with the exception of those from China were predominantly of the csLV34a type. Only one size variant, csLV34a, was detected among the diploid and polyploid D genome-containing species, indicating that csLV34b arose subsequent to hexaploid bread wheat synthesis. The lineage of the csLV34b allele associated with Lr34/Yr18 in modern wheat cultivars from North and South America, CIMMYT, Australia, and Russia was tracked back to the cultivars Mentana and Ardito developed in Italy by Nazareno Strampelli in the early 1900s. The robustness of the csLV34 marker in postulating the likely occurrence of Lr34/Yr18 across a wide range of wheat germplasm and its utility in wheat breeding was confirmed.