Study of the SH3-domain GRB2-like 2 gene expression in laryngeal carcinoma
Study of the SH3-domain GRB2-like 2 gene expression in laryngeal carcinoma
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DOI：
10.1097/00029330-200703010-00007
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发表时间：
2007-03-05
影响因子：
6.1
通讯作者：
Sun Kai-lai
中科院分区：
文献类型：
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作者：
Shang Chao;Fu Wei-neng;Sun Kai-lai
背景喉癌是上呼吸道常见的恶性肿瘤，95%的病例为喉鳞状细胞癌（Laryngeal squamous cell carcinoma，LSCC）。SH 3-domain GRB 2-like 2（SH 3GL 2）基因在喉鳞状细胞癌中表达缺失。方法采用Real-time PCR、免疫组化和Western blotting方法检测SH 3GL 2基因在喉鳞癌组织中的表达，分析SH 3GL 2基因在喉鳞癌组织中的表达变化规律，探讨SH 3GL 2基因在喉鳞癌中的表达与喉鳞癌的关系。免疫组化结果显示SH 3GL 2蛋白主要定位于胞浆，其阳性细胞率及表达水平随喉鳞癌病理分级而波动; Western blotting结果显示SH 3GL 2蛋白在喉鳞癌组织中表达显著下调，结论SH 3GL 2是喉鳞癌的相关基因，其表达水平随病理分级而波动，提示SH 3GL 2参与了喉鳞癌的发生发展和LSCC的进展。它有望成为一种新的肿瘤标志物，为寻找新的抑癌基因和喉癌侵袭转移的相关因素提供新的思路。
Background Laryngeal carcinoma is a common malignant tumor of the upper respiratory tract, and in 95% of cases the tumor is laryngeal squamous cell carcinoma (LSCC). The abnormity of SH3-domain GRB2-like 2 (SH3GL2) gene was found in LSCC. In order to clarify the relationship between SH3GL2 gene and LSCC, we evaluated the expression of the SH3GL2 gene in LSCC.Method Real-time PCR, immunohistochemistry and Western blotting were used to detect the mRNA and protein expression and find the various rules of SH3GL2 gene in LSCC.Results The result of real-time PCR showed that the expression level of SH3GL2 mRNA in LSCC tissue was apparently down-regulated; immunohistochemical analysis showed that SH3GL2 protein was mainly located in cytoplasm, the rate of positive cells and SH3GL2 protein expression level were fluctuated with the pathological classification of LSCC; the result of Western blotting showed that SH3GL2 protein was down-regulated significantly in LSCC samples, especially in metastatic lymph nodes.Conclusions These results suggest that SH3GL2 is a LSCC related gene and its expression level is fluctuated with the pathological classification which indicate that SH3GL2 participates in the development and progression of LSCC. And it may be considered as a novel tumor marker to find both a new anti-oncogene and relative factors of invasion and metastasis of laryngeal carcinoma.