Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo
Bovine AAV transcytosis inhibition by tannic acid results in functional expression of CFTR in vitro and altered biodistribution in vivo
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DOI：
10.1038/gt.2011.138
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发表时间：
2012-05-01
期刊：
影响因子：
5.1
通讯作者：
Chiorini, J. A.
中科院分区：
文献类型：
--
作者：
Di Pasquale, G.;Ostedgaard, L.;Chiorini, J. A.
牛腺相关病毒(BAAV)可通过转导或转导途径进入细胞。我们先前证明，通过跨细胞途径进入的颗粒可以通过单宁酸(TA)阻止颗粒胞吐来重定向转导细胞。为了研究这种方法在肺基因治疗中是否有用，我们在体外和体内测试了TA对囊性纤维化气道上皮细胞和小鼠肺组织中BAAV转导的影响。我们的研究结果表明，BAAV可以在体内发生转胞作用，TA治疗减少了转胞作用，增加了肺转导。TA处理不影响BAAV表达的囊性纤维化跨膜调节蛋白的分选和活性。基因治疗(2012年)19576-581；DOI：10.1038/gt.2011.138；2011年10月20日在线发布
Bovine adeno-associated virus (BAAV) can enter a cell either through a transcytosis or transduction pathway. We previously demonstrated that particles entering via the transcytosis pathway can be redirected to transduce the cell by blocking particle exocytosis with tannic acid (TA). To investigate whether this approach is useful in lung gene therapy applications, we tested the effect of TA on BAAV transduction in cystic fibrosis airway epithelia in vitro, and in mouse lung in vivo. Our findings suggest that BAAV transcytosis can occur in vivo and that treatment with TA reduces transcytosis and increases lung transduction. TA treatment did not impair the sorting and the activity of the BAAV expressed cystic fibrosis transmembrane regulator membrane protein. Gene Therapy (2012) 19, 576-581; doi:10.1038/gt.2011.138; published online 20 October 2011