RATTLESNAKE STRUCTURE OF A FILAMENTOUS PLANT RNA VIRUS BUILT OF 2 CAPSID PROTEINS
RATTLESNAKE STRUCTURE OF A FILAMENTOUS PLANT RNA VIRUS BUILT OF 2 CAPSID PROTEINS
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DOI：
10.1073/pnas.92.7.2470
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发表时间：
1995-03-28
影响因子：
11.1
通讯作者：
ATABEKOV, JG
中科院分区：
文献类型：
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作者：
AGRANOVSKY, AA;LESEMANN, DE;ATABEKOV, JG
植物简单RNA病毒的细长颗粒由包被大量相同衣壳蛋白亚基的RNA分子组成，形成规则的螺旋结构，烟草花叶病毒是其原型。据报道，闭锁病毒甜菜黄化病毒(BYV)的丝状颗粒含有大约4000个相同的22 kDa(PIT)衣壳蛋白亚基。BYV基因组编码一个24 kDa的蛋白(P24)，在结构上与p22相关。我们用免疫电子显微镜和针对重组p24蛋白及其N端肽的特异性抗体在BYV颗粒中寻找p24，发现在1370 nm丝状病毒颗粒一端的75 nm片段被两种类型的抗体一致标记，从而表明p24确实是第二衣壳蛋白，并且与其他具有螺旋对称性的植物病毒不同，闭锁病毒颗粒具有“响尾蛇”结构而不是均匀的结构。
Elongated particles of simple RNA viruses of plants are composed of an RNA molecule coated with numerous identical capsid protein subunits to form a regular helical structure, of which tobacco mosaic virus is the archetype. Filamentous particles of the closterovirus beet yellows virus (BYV) reportedly contain approximate to 4000 identical 22-kDa (pit) capsid protein subunits. The BYV genome encodes a 24-kDa protein (p24) that is structurally related to the p22. We searched for the p24 in BYV particles by using immunoelectron microscopy with specific antibodies against the recombinant p24 protein and its N-terminal peptide, A 75-nm segment at one end of the 1370-nm filamentous viral particle was found to be consistently labeled with both types of antibodies, thus indicating that p24 is indeed the second capsid protein and that the closterovirus particle, unlike those of other plant viruses with helical symmetry, has a ''rattlesnake'' rather than uniform structure.