Distribution of nestin immunoreactivity in the normal adult human forebrain
Distribution of nestin immunoreactivity in the normal adult human forebrain
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DOI：
10.1016/s0006-8993(02)02615-x
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发表时间：
2002-07-12
期刊：
影响因子：
2.9
通讯作者：
Yao, ZB
中科院分区：
文献类型：
--
作者：
Gu, HY;Wang, SX;Yao, ZB
使用新型抗人巢蛋白小鼠单克隆和兔多克隆抗血清研究了整个正常成人前脑中巢蛋白免疫反应性的分布。巢蛋白免疫反应细胞根据其形态特征可分为三种类型。第一类为神经元样巢蛋白免疫反应细胞，分布于海马CA1-3、隔、斜带核、杏仁核和Meynert基底核。第二种为星形胶质细胞样细胞，分布于室管膜下区和齿状回颗粒下层。第三类细胞胞体较小，突起较少，也分布于室管膜下区和齿状回颗粒下层。免疫组化双重染色显示，斜带核和海马巢蛋白阳性、神经元样细胞也表达NSE。然而，室管膜下区和齿状回颗粒下层的星形胶质细胞样巢蛋白免疫反应细胞没有被 GFAP 双标记。尽管漏斗部分布有一些巢蛋白免疫反应性纤维，但在皮质中未检测到巢蛋白免疫反应性细胞。这些数据表明巢蛋白存在于成人大脑的室管膜下区和齿状回之外，也意味着巢蛋白免疫反应细胞可能在基础前脑功能的调节中发挥作用。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The distribution of nestin immunoreactivity was studied in the whole normal adult human forebrains using new anti-human nestin mouse monoclonal and rabbit polyclonal antiserum. The nestin immunoreactive cells could be divided into three types according to their morphological characteristics. The first type contained neuron-like nestin immunoreactive cells, distributed in CA1-3 of hippocampus, septum, the nucleus of diagonal band, amygdala and basal nucleus of Meynert. The second type contained astrocyte-like cells, distributed in the subependymal zone and subgranular layer of dentate gyrus. The third type of cells had smaller cell bodies and fewer processes, also distributed in the subependymal zone and subgranular layer of dentate gyrus. Double immunohistochemical staining showed that the nestin positive, neuron-like cells in the nucleus of diagonal band and hippocampus also expressed NSE. However, the astrocyte-like nestin immunoreactive cells of the subependymal zone and subgranular layer of dentate gyrus were not double labeled with GFAP. Although some nestin immunoreactive fibers were distributed in the infundibulum, no nestin-immunoreactive cells were detected in the cortex. These data indicate that nestin exist in the adult human brain outside of the subependymal zone and dentate gyrus and also implies that nestin-immunoreactive cells may play a role in the modulation of basal forebrain function. (C) 2002 Elsevier Science B.V. All rights reserved.