DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS INTO SCHWANN CELL PHENOTYPE IN COMPARISON WITH BONE MARROW STEM CELLS
DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS INTO SCHWANN CELL PHENOTYPE IN COMPARISON WITH BONE MARROW STEM CELLS
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DOI：
10.22038/ijbms.2010.5089
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发表时间：
2010-07
影响因子：
2.2
通讯作者：
Zolikha Golipoor;I. Kashani;M. Akbari;G. Hassanzadeh;F. Malek;R. Mahmoudi
中科院分区：
文献类型：
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作者：
Zolikha Golipoor;I. Kashani;M. Akbari;G. Hassanzadeh;F. Malek;R. Mahmoudi
目的：骨髓是人类多能间充质干细胞（MSCs）的传统来源，但脂肪组织似乎是另一种更容易获得的来源。本研究将大鼠脂肪源性干细胞（ADSCs）诱导分化为雪旺样细胞，并与大鼠骨髓干细胞（BMSCs）比较其向雪旺样细胞分化的潜力。材料与方法研究骨髓间充质干细胞和ADSCs的特异性标志物表达、成骨分化和成脂分化。将其诱导分化为雪旺样细胞，并分析雪旺特异性标记物的表达。免疫细胞化学分化标志物为S-100，实时定量实时聚合酶链反应（RT-PCR）标志物为S100、P75和胶质纤维酸性蛋白（GFAP）。采用3-（4,5 -二甲基噻唑-2 -基）- 2,5 -二苯基溴化四唑（MTT）法和Annexin v -异硫氰酸荧光素(FITC)/碘化丙啶（PI）双标记法检测细胞早期凋亡。结果BMSCs与ADSCs在msc特异性标志物的表达、成骨分化和成脂分化方面具有相似性。定量RT-PCR和免疫细胞化学分析表明，骨髓间充质干细胞和ADSCs在雪旺样细胞分化后具有相同的雪旺特异性标志物表达。然而，P75基因在骨髓间充质干细胞中的表达高于ADSCs。MTT实验和流式细胞术发现，培养基中总BMSCs和ADSCs中，20% - 30%的细胞死亡，但剩余的细胞群仍然强烈附着在底物上并分化。结论与骨髓间充质干细胞相比，ADSCs的雪旺样细胞分化能力略有下降。因此，BMSCs比ADSCs更适合用于组织工程。
Objective(s) Bone marrow is the traditional source of human multipotent mesenchymal stem cells (MSCs), but adipose tissue appears to be an alternative and more readily available source. In this study, rat adipose-derived stem cells (ADSCs) were induced to differentiate into Schwann-like cells and compared with rat bone marrow stem cells (BMSCs) for their Schwann-like cells differentiation potential. Materials and Methods BMSCs and ADSCs were characterized for expression of MSCs-specific markers, osteogenic and adipogenic differentiation. They were induced to differentiate into Schwann-like cells and analyzed for expression of the Schwann specific markers. The immunocytochemical differentiation markers were S-100 and real time quantitative Real-time polymerase chain reaction (RT-PCR) markers were S100, P75 and glial fibrillary acidic protein (GFAP). 3-(4, 5-Dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and Annexin V-Fluorescein isothiocyanate (FITC)/ Propidium iodide (PI) double labeling method were employed to detect early stage cell apoptosis. Results BMSCs and ADSCs showed similarities in expression of the MSC-specific markers, osteogenic and adipogenic differentiation. Both quantitative RT-PCR and immunocytochemical analysis demonstrated that BMSCs and ADSCs had equal expression of the Schwann-specific markers following Schwann-like cells differentiation. However, gene expression of P75 was higher in BMSCs compared with ADSCs. MTT assay and flow cytometry found that of the total BMSCs and ADSCs in the culture medium, 20% to 30% of the cells died, but the remaining cell population remained strongly attached to the substrate and differentiated. Conclusion Comparative analysis showed that Schwann-like cell differentiation potential of ADSCs was slightly decreased in comparison with BMSCs. Therefore, BMSCs are more favorable choice than ADSCs for tissue engineering.