An expanded genetic code in mammalian cells with a functional quadruplet codon.
An expanded genetic code in mammalian cells with a functional quadruplet codon.
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DOI：
10.1021/cb4001662
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发表时间：
2013-07-19
影响因子：
4
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Niu W;Schultz PG;Guo J
我们已经利用体外进化来鉴定tRNA变体，其在细菌和哺乳动物细胞中响应于四联体密码子而将非天然氨基酸掺入蛋白质中的活性显著增强。这种方法将有助于创建一个优化和标准化的系统，用于使用四联体密码子遗传掺入非天然氨基酸，这将允许生物合成含有多个非天然结构单元的生物聚合物。
We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells. This approach will facilitate the creation of an optimized and standardized system for the genetic incorporation of unnatural amino acids using quadruplet codons, which will allow the biosynthesis of biopolymers that contain multiple unnatural building blocks.