CAFFEINE INJECTION RAISES BRAIN TRYPTOPHAN LEVEL, BUT DOES NOT STIMULATE THE RATE OF SEROTONIN SYNTHESIS IN RAT-BRAIN
CAFFEINE INJECTION RAISES BRAIN TRYPTOPHAN LEVEL, BUT DOES NOT STIMULATE THE RATE OF SEROTONIN SYNTHESIS IN RAT-BRAIN
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DOI：
10.1016/0024-3205(84)90094-8
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发表时间：
1984-01-01
期刊：
影响因子：
6.1
通讯作者：
FERNSTROM, JD
中科院分区：
文献类型：
--
作者：
FERNSTROM, MH;BAZIL, CW;FERNSTROM, JD
急性咖啡因注射(100 mg/kg)会提高大脑中Trp(Trp)、5-羟色胺(5-HT)和5-羟基吲哚乙酸(5HIAA)的水平。实验确定5-羟色胺和5-羟色胺的增加是否是由于刺激了5-羟色胺的合成速率。在体内测量了注射NSD-1015后5-羟色氨酸(5HTP)的蓄积率和~3H-TRY合成~3H-5-羟吲哚的速率，发现咖啡因的作用是正常的。体外测定的脑匀浆色氨酸羟基酶活性也不受咖啡因的影响。咖啡因引起的脑内5-羟色胺和5HIAA水平的升高显然并不反映5-羟色胺合成的增强，尽管大脑色氨酸水平显著升高。因此，一定有其他机制(S)对大脑5-羟基吲哚水平的升高负责。
Acute caffeine injection (100 mg/kg) elevates brain levels of Try (TRP), serotonin (5HT) and 5-hydroxyindoleacetic acid (5HIAA). Experiments were performed to determine if the increases in 5HT and 5HIAA result from a stimulation of the rate of 5HT synthesis. Both the rate of 5-hydroxytryptophan (5HTP) accumulation following NSD-1015 injection, and the rate of 3H-5-hydroxyindole synthesis from 3H-Try were measured in vivo following caffeine administration and found to be normal. Tryptophan hydroxylase activity, as measured in vitro in brain homogenates, was also unaffected by caffeine. The elevations in brain 5HT and 5HIAA levels produced by caffeine apparently do not reflect enhanced 5HT synthesis, despite significant elevations in brain TRP level. Some other mechanism(s) must therefore be responsible for these elevations in brain 5-hydroxyindole levels.