P13SUC1 ACTS IN THE FISSION YEAST-CELL DIVISION CYCLE AS A COMPONENT OF THE P34CDC2 PROTEIN-KINASE
P13SUC1 ACTS IN THE FISSION YEAST-CELL DIVISION CYCLE AS A COMPONENT OF THE P34CDC2 PROTEIN-KINASE
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DOI：
10.1002/j.1460-2075.1987.tb02676.x
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发表时间：
1987-11-01
期刊：
影响因子：
11.4
通讯作者：
BEACH, D
中科院分区：
文献类型：
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作者：
BRIZUELA, L;DRAETTA, G;BEACH, D
cdc2+编码分裂酵母细胞分裂周期G1和G2期所需的蛋白激酶。Suc1+是一种必需基因，最初被鉴定为质粒携带的序列，其可以拯救某些温度敏感的CDC2突变体。为了研究suc1+基因产物在细胞周期中的作用，在大肠杆菌中表达了p13suc1并纯化。制备了免疫亲和纯化的抗p13suc1多克隆血清，并用于鉴定裂殖酵母中的p13suc1。该蛋白的丰度在细胞周期期间或进入稳定期期间都没有改变。在酵母裂解物中发现p13suc1与cdc2+基因产物形成复合物。大约5%的细胞p34cdc2与p13suc1相关，并且这部分p34cdc2作为蛋白激酶是有活性的。在携带cdc2温度敏感等位基因的酵母菌株中，复合物的稳定性被破坏，这些等位基因可被suc1+的过表达抑制。p13suc1和p34cdc2之间的关联水平不受细胞周期阻滞在不利的营养条件。p13suc1不是p34cdc2蛋白激酶的底物。相反，我们建议，它作为一个调节组件的p34cdc2，促进与其他蛋白质的相互作用。
cdc2+ encodes a protein kinase that is required during both G1 and G2 phases of the cell division cycle in fission yeast. suc1+ is an essential gene that was originally identified as a plasmid-borne sequence that could rescue certain temperature-sensitive cdc2 mutants. To investigate the role of the suc1+ gene product in the cell cycle p13suc1 has been expressed in Escherichia coli and purified. An immunoaffinity purified anti-p13suc1 polyclonal serum has been prepared and used to identify p13suc1 in fission yeast. The abundance of this protein did not alter either during the cell cycle or during entry into stationary phase. p13suc1 was found in yeast lysates in a complex with the cdc2+ gene product. Approximately 5% of cellular p34cdc2 was associated with p13suc1, and this fraction of p34cdc2 was active as a protein kinase. The stability of the complex was disrupted in yeast strains carrying temperature-sensitive alleles of cdc2 that are suppressible by overexpression of suc1+. The level of association between p13suc1 and p34cdc2 was not affected by cell cycle arrest in adverse nutritional conditions. p13suc1 is not a substrate of the p34cdc2 protein kinase. We propose instead that it acts as a regulatory component of p34cdc2 that facilitates interaction with other proteins.